Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   U719_RS00350 Genome accession   NC_022794
Coordinates   64262..65638 (+) Length   458 a.a.
NCBI ID   WP_029343076.1    Uniprot ID   -
Organism   Exiguobacterium sp. MH3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 59262..70638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U719_RS00330 (U719_00330) - 59763..60218 (+) 456 WP_023466578.1 CtsR family transcriptional regulator -
  U719_RS00335 (U719_00335) - 60244..60672 (+) 429 WP_023466580.1 hypothetical protein -
  U719_RS00340 (U719_00340) - 60665..61738 (+) 1074 WP_023466586.1 ATP--guanido phosphotransferase -
  U719_RS00345 (U719_00345) clpC 61738..64185 (+) 2448 WP_023466588.1 ATP-dependent protease ATP-binding subunit ClpC -
  U719_RS00350 (U719_00350) radA 64262..65638 (+) 1377 WP_029343076.1 DNA repair protein RadA Machinery gene
  U719_RS00355 (U719_00355) - 65766..66851 (+) 1086 WP_023466590.1 PIN/TRAM domain-containing protein -
  U719_RS00360 (U719_00360) ispD 66984..67667 (+) 684 WP_023466592.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  U719_RS00365 (U719_00365) ispF 67670..68152 (+) 483 WP_029343079.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  U719_RS00370 (U719_00370) gltX 68196..69644 (+) 1449 WP_023466595.1 glutamate--tRNA ligase -
  U719_RS00375 (U719_00375) epsC 69872..70591 (+) 720 WP_255343794.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50039.64 Da        Isoelectric Point: 6.6525

>NTDB_id=63485 U719_RS00350 WP_029343076.1 64262..65638(+) (radA) [Exiguobacterium sp. MH3]
MAKLKTKFVCQSCGTESPKWMGRCSGCGEWNTMVEEVVEEKKGRRGAAFVHTTTKQLKPERLANIVSQEESRVFTGSGEF
DRVLGGGIVPGSMVLVGGDPGIGKSTILLQTSARLAQRGEKVLYISGEESLKQTKLRAERLGLPTQDLFVLSETDMNMIE
RVVDEEQPRFLIIDSIQTVYIDEIQSAPGSVTQVRECTAMLMKIAKSRGIAIFIVGHVTKQGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRAVKNRFGSTNEIGIFEMRESGLEEVLNPSEIFLEERTSGVSGSTIVASMEGTRTVLVELQALISPTSF
GNPRRMATGIDQNKVALLMAVLEKRSGLLLQTQDAYLKAAGGVKLDEPAIDLAVCVAIASSFRDKPTRPTDVVIGEVGLT
GEVRRVSRIEQRVAEAAKLGFTRAIIPKNNLGGWTYPDGITVVGVESVDEALRETIPF

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=63485 U719_RS00350 WP_029343076.1 64262..65638(+) (radA) [Exiguobacterium sp. MH3]
TTGGCTAAGTTAAAAACAAAATTCGTTTGTCAAAGCTGTGGGACCGAATCACCGAAATGGATGGGACGTTGTTCTGGATG
CGGAGAATGGAACACGATGGTCGAAGAAGTCGTCGAAGAGAAAAAAGGGCGGCGCGGTGCCGCTTTCGTTCATACGACAA
CAAAACAATTGAAGCCAGAGCGCCTCGCGAACATCGTCTCGCAAGAAGAGAGTCGCGTCTTTACCGGAAGTGGTGAATTC
GACCGTGTCCTCGGAGGCGGTATCGTTCCAGGTTCGATGGTGCTTGTTGGAGGAGATCCGGGGATCGGGAAGTCGACGAT
CTTACTGCAGACGAGCGCACGTCTTGCACAACGTGGCGAAAAAGTGCTCTACATCTCAGGAGAAGAATCGCTCAAACAAA
CGAAATTACGCGCGGAACGGCTTGGCTTACCGACACAGGATTTGTTCGTCCTGAGTGAGACGGATATGAACATGATTGAA
CGTGTCGTCGATGAAGAACAGCCACGTTTTTTAATCATCGACTCGATTCAAACCGTCTACATCGATGAAATCCAGTCTGC
ACCCGGGAGCGTGACACAGGTACGTGAATGTACGGCGATGCTGATGAAAATTGCGAAGAGTCGCGGTATTGCGATCTTCA
TCGTCGGTCACGTCACGAAACAGGGTTCGATTGCTGGACCACGTTTACTGGAGCACATGGTGGATGCTGTCTTGTATTTC
GAAGGCGAACGTCACCATACGTTCCGGATTCTGCGTGCCGTCAAAAACCGATTTGGTTCAACGAATGAGATCGGAATTTT
TGAGATGCGGGAGTCTGGTCTTGAAGAGGTCTTGAATCCGTCAGAAATTTTCCTCGAAGAGCGGACATCCGGTGTATCCG
GTTCGACGATTGTCGCTTCCATGGAAGGGACACGGACCGTCCTTGTCGAACTGCAAGCACTGATTTCACCGACATCATTC
GGGAATCCAAGACGGATGGCGACCGGAATCGATCAAAATAAGGTGGCCTTATTGATGGCTGTTCTTGAGAAACGGTCGGG
TCTTCTCCTTCAGACACAAGATGCGTACCTCAAGGCAGCCGGGGGTGTGAAGCTGGATGAACCAGCGATTGACTTGGCGG
TTTGTGTGGCAATTGCTTCTAGTTTCCGTGACAAACCGACGCGTCCGACGGATGTCGTGATTGGTGAGGTCGGATTGACA
GGTGAGGTTCGCCGTGTATCGCGGATTGAACAACGCGTAGCAGAAGCAGCTAAACTCGGTTTCACGCGGGCGATCATTCC
GAAAAACAACTTAGGTGGTTGGACGTATCCAGACGGAATTACGGTCGTCGGTGTCGAGAGTGTGGACGAAGCATTACGTG
AAACGATTCCGTTTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.588

99.563

0.723
  radA Streptococcus pneumoniae Rx1

62.775

99.127

0.622
  radA Streptococcus pneumoniae D39

62.775

99.127

0.622
  radA Streptococcus pneumoniae R6

62.775

99.127

0.622
  radA Streptococcus pneumoniae TIGR4

62.775

99.127

0.622
  radA Streptococcus mitis NCTC 12261

62.775

99.127

0.622
  radA Streptococcus mitis SK321

62.775

99.127

0.622

Multiple sequence alignment