Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LSO60_RS13180 Genome accession   NZ_CP089051
Coordinates   2771922..2772674 (-) Length   250 a.a.
NCBI ID   WP_263513406.1    Uniprot ID   A0AA46P3S8
Organism   Acinetobacter ursingii strain RIVM_C010559     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2766922..2777674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO60_RS13160 (LSO60_13135) - 2767979..2768680 (-) 702 WP_004992686.1 tetratricopeptide repeat protein -
  LSO60_RS13165 (LSO60_13140) hisS 2768710..2770002 (-) 1293 WP_049174910.1 histidine--tRNA ligase -
  LSO60_RS13170 (LSO60_13145) ispG 2770006..2771124 (-) 1119 WP_004992682.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LSO60_RS13175 (LSO60_13150) - 2771149..2771931 (-) 783 WP_263512384.1 helix-turn-helix domain-containing protein -
  LSO60_RS13180 (LSO60_13155) pilF 2771922..2772674 (-) 753 WP_263513406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LSO60_RS13185 (LSO60_13160) rlmN 2772742..2773968 (-) 1227 WP_125269616.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LSO60_RS13190 (LSO60_13165) ndk 2774090..2774521 (-) 432 WP_004994861.1 nucleoside-diphosphate kinase -
  LSO60_RS13195 (LSO60_13170) iscX 2774633..2774833 (-) 201 WP_004992669.1 Fe-S cluster assembly protein IscX -
  LSO60_RS13200 (LSO60_13175) - 2774938..2775489 (-) 552 WP_263512385.1 phosphatase PAP2 family protein -
  LSO60_RS13205 (LSO60_13180) - 2775519..2776796 (-) 1278 WP_263512386.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28322.16 Da        Isoelectric Point: 9.5324

>NTDB_id=634821 LSO60_RS13180 WP_263513406.1 2771922..2772674(-) (pilF) [Acinetobacter ursingii strain RIVM_C010559]
MALLLTGCQSQKTTKDPKKAVQVRTQLAAEYIKSGDLDAAKRALDQALEVNSRDSMANMMMGVLLQQEGSATNLEKADTY
FKRAISEEPTNAQARNNYGTYLYQLKRYNDAIEQFTIAGATLGYDQRYRALENLGRIYVLLGDRVNAEKSFKQALQANRD
SYVSMLELSEIFYLQQQMPAATQMYGQYVRTVGQTNQGARALWVGIRVARANGDRMGMQALINQLRALFPDSSEYKRYLQ
LQYSTEAVWK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=634821 LSO60_RS13180 WP_263513406.1 2771922..2772674(-) (pilF) [Acinetobacter ursingii strain RIVM_C010559]
ATGGCGTTGCTGTTAACGGGATGCCAATCTCAAAAAACAACCAAAGACCCTAAAAAAGCGGTGCAAGTCCGTACACAACT
TGCAGCTGAATATATTAAGTCTGGTGATCTGGATGCTGCTAAACGCGCATTGGATCAGGCTTTGGAAGTGAACTCGCGTG
ACTCCATGGCCAATATGATGATGGGTGTACTCTTACAACAAGAAGGTAGTGCAACAAATCTCGAGAAAGCAGACACGTAT
TTTAAACGTGCAATCTCGGAAGAGCCTACCAATGCACAAGCTCGTAATAATTATGGTACGTACTTATACCAATTGAAACG
TTATAATGACGCGATTGAACAATTTACGATTGCAGGGGCAACACTTGGGTATGATCAGCGTTATCGTGCCCTTGAAAATC
TGGGGCGTATCTACGTATTGCTTGGCGATCGTGTGAATGCTGAAAAGTCATTCAAACAAGCTTTACAGGCAAACCGCGAC
TCTTATGTATCTATGTTAGAGTTGTCAGAGATTTTTTATTTGCAACAGCAAATGCCAGCGGCGACTCAAATGTATGGACA
ATATGTGCGTACAGTGGGGCAAACCAATCAGGGGGCGCGTGCACTTTGGGTGGGGATTCGTGTTGCACGAGCCAATGGTG
ATCGAATGGGAATGCAGGCACTGATCAATCAACTGCGTGCTTTATTCCCAGATAGTTCAGAATATAAACGTTATTTGCAA
TTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(23-52)

Tetratricopeptide repeat (TPR_8)

(129-160)

Tetratricopeptide repeat (TPR_17)

(81-113)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

80.876

100

0.812
  pilF Acinetobacter baylyi ADP1

76.707

99.6

0.764