Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LSO59_RS13750 Genome accession   NZ_CP089038
Coordinates   2948587..2949705 (+) Length   372 a.a.
NCBI ID   WP_004990996.1    Uniprot ID   A0A3F3L4S7
Organism   Acinetobacter ursingii strain RIVM_C011601     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2943587..2954705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO59_RS13725 (LSO59_13700) - 2944201..2944986 (+) 786 WP_004990984.1 transglutaminase-like domain-containing protein -
  LSO59_RS13730 (LSO59_13705) - 2945067..2945758 (+) 692 Protein_2688 HAD family hydrolase -
  LSO59_RS13735 (LSO59_13710) - 2945716..2946561 (-) 846 WP_004990991.1 undecaprenyl-diphosphate phosphatase -
  LSO59_RS13740 (LSO59_13715) - 2946712..2947398 (-) 687 WP_104794966.1 YggS family pyridoxal phosphate-dependent enzyme -
  LSO59_RS13745 (LSO59_13720) pilT 2947522..2948559 (+) 1038 WP_104794965.1 type IV pilus twitching motility protein PilT Machinery gene
  LSO59_RS13750 (LSO59_13725) pilU 2948587..2949705 (+) 1119 WP_004990996.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LSO59_RS13755 (LSO59_13730) fur 2949779..2950219 (-) 441 WP_004990998.1 ferric iron uptake transcriptional regulator -
  LSO59_RS13760 (LSO59_13735) - 2950331..2950729 (+) 399 WP_004991001.1 outer membrane protein assembly factor BamE -
  LSO59_RS13765 (LSO59_13740) - 2950773..2951093 (-) 321 WP_104794964.1 RnfH family protein -
  LSO59_RS13770 (LSO59_13745) - 2951090..2952175 (-) 1086 WP_104794963.1 hypothetical protein -
  LSO59_RS13775 (LSO59_13750) - 2952338..2952787 (-) 450 WP_004991008.1 bacteriohemerythrin -
  LSO59_RS13780 (LSO59_13755) - 2953123..2953971 (-) 849 WP_010588903.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41822.00 Da        Isoelectric Point: 6.0604

>NTDB_id=634743 LSO59_RS13750 WP_004990996.1 2948587..2949705(+) (pilU) [Acinetobacter ursingii strain RIVM_C011601]
MDFNDLLNLMIEQQASDLFVTAGVEPSMKINGQIVPVSKTKLNAEVVGQLIQSIMSDKQRKEFDESRECNFAIVNREKTA
RFRVSAFQQRDMPGMVLRRIETIIPTMDELRLPPILKDLAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITVE
DPIEFIHEHAGCIVTQREVGIDTDSFDVALKNTLRQAPDVILIGEIRSREVMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFESDRHNQLYMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLSIKLSEEGAEQLLNASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=634743 LSO59_RS13750 WP_004990996.1 2948587..2949705(+) (pilU) [Acinetobacter ursingii strain RIVM_C011601]
ATGGATTTTAATGACCTACTCAATCTAATGATTGAACAACAAGCATCAGACCTGTTTGTCACCGCAGGTGTGGAACCTTC
GATGAAAATTAATGGTCAGATTGTCCCAGTGTCTAAGACTAAGCTGAATGCTGAGGTCGTTGGACAACTGATTCAGTCCA
TTATGTCTGATAAGCAACGCAAGGAATTTGACGAGAGCAGAGAGTGTAACTTTGCGATTGTAAATCGGGAAAAAACCGCA
CGTTTTCGTGTCAGTGCATTTCAGCAGCGTGATATGCCAGGCATGGTATTACGTCGTATCGAAACGATTATCCCTACAAT
GGATGAATTACGCTTACCGCCCATTCTTAAAGATCTGGCCATGACCAAACGTGGCATTATTATCTTTGTTGGGGCAACAG
GTACGGGTAAATCCACTTCATTGGCATCCATGATTGGTTATCGAAATCAGAACTCCAAAGGTCATATCATCACGGTTGAA
GATCCAATCGAGTTCATTCATGAACACGCAGGTTGTATTGTGACCCAACGTGAGGTCGGTATTGATACAGACTCTTTCGA
TGTGGCCCTGAAAAACACACTACGACAAGCCCCTGATGTGATTTTGATTGGTGAGATTCGTTCACGTGAGGTCATGGACT
ATGCCATTGCCTTTGCTGAAACAGGACATCTGGTCTTAGCGACATTACACGCCAACAACGCCAACCAAGCATTGGATCGT
ATTATTCATTTCTTTGAAAGTGATCGCCATAATCAGCTCTATATGGATCTCTCGCTCAATCTAAAAGCCATGGTGGCACA
GCAATTGATCCCAACGCCAGATGGCAATTCACGTCGTGCTGCGATTGAAATATTGATCAATACACCATTACTTGCTGACT
ATATTCGTAAAGGTGAAATTCATGAAATCAAAGATTTAATGAAACGTTCACGTGAACTCGGCATGCAAACCTTTGATCAG
GCTTTATTTGATCTTTATAAAGCAGGTCAAATTACCTATAAAGATGCCTTAAAACATGCCGACTCGCCAAACGATCTACG
TCTCAGTATCAAACTTTCAGAGGAAGGTGCAGAACAACTGCTGAACGCCAGTCGCAACATTACTTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3F3L4S7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.984

100

0.782
  pilU Pseudomonas stutzeri DSM 10701

69.811

99.731

0.696
  pilU Vibrio cholerae strain A1552

55.84

94.355

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.484

97.849

0.406
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter baylyi ADP1

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36