Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LSO59_RS03425 Genome accession   NZ_CP089038
Coordinates   692254..693006 (+) Length   250 a.a.
NCBI ID   WP_004992677.1    Uniprot ID   A0AA46NZF8
Organism   Acinetobacter ursingii strain RIVM_C011601     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 687254..698006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO59_RS03400 (LSO59_03395) - 688132..689409 (+) 1278 WP_004992664.1 glycosyltransferase family 4 protein -
  LSO59_RS03405 (LSO59_03400) - 689439..689990 (+) 552 WP_004992665.1 phosphatase PAP2 family protein -
  LSO59_RS03410 (LSO59_03405) iscX 690095..690295 (+) 201 WP_004992669.1 Fe-S cluster assembly protein IscX -
  LSO59_RS03415 (LSO59_03410) ndk 690407..690838 (+) 432 WP_004992670.1 nucleoside-diphosphate kinase -
  LSO59_RS03420 (LSO59_03415) rlmN 690960..692186 (+) 1227 WP_004992674.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LSO59_RS03425 (LSO59_03420) pilF 692254..693006 (+) 753 WP_004992677.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LSO59_RS03430 (LSO59_03425) - 692997..693779 (+) 783 WP_004992678.1 helix-turn-helix domain-containing protein -
  LSO59_RS03435 (LSO59_03430) ispG 693804..694922 (+) 1119 WP_004992682.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LSO59_RS03440 (LSO59_03435) hisS 694926..696218 (+) 1293 WP_004992685.1 histidine--tRNA ligase -
  LSO59_RS03445 (LSO59_03440) - 696248..696949 (+) 702 WP_004992686.1 YfgM family protein -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28407.31 Da        Isoelectric Point: 9.3924

>NTDB_id=634732 LSO59_RS03425 WP_004992677.1 692254..693006(+) (pilF) [Acinetobacter ursingii strain RIVM_C011601]
MVLLLTGCQSQKTTKDPKKAVQVRTQLAAEYIKSGDLDAAKRALDQALEVNSRDSMANMMMGVLLQQEGSATNLEKADTY
FKRAISEEPTNAQARNNYGTYLYQLKRYNDAIEQFTIAGATLGYDQRYRALENLGRIYVLLGDRVNAEKSFKQALQANRD
SYVSMLELSEIFYLQQQMPAATQMYEQYVRTVGQTNQGARALWVGIRVARANGDRMGMLALINQLRALFPDSSEYKRYLQ
LQYSTEAVWK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=634732 LSO59_RS03425 WP_004992677.1 692254..693006(+) (pilF) [Acinetobacter ursingii strain RIVM_C011601]
ATGGTGTTGCTGTTAACGGGATGCCAATCTCAAAAAACAACCAAAGACCCTAAAAAAGCGGTGCAGGTCCGTACACAACT
TGCAGCTGAATATATCAAGTCTGGTGATCTGGATGCTGCTAAACGCGCATTGGATCAGGCTTTGGAAGTGAACTCGCGTG
ACTCCATGGCCAATATGATGATGGGTGTACTCTTACAACAAGAAGGTAGTGCAACAAATCTCGAGAAAGCAGACACGTAT
TTTAAACGTGCAATCTCGGAAGAACCTACCAATGCACAAGCTCGTAATAATTATGGTACGTACTTATACCAATTGAAACG
TTATAATGACGCGATTGAACAATTTACGATTGCAGGGGCAACACTTGGGTATGATCAGCGTTATCGTGCCCTTGAAAATC
TGGGGCGTATCTACGTATTGCTTGGCGATCGTGTGAATGCTGAAAAGTCATTCAAACAAGCTTTACAGGCAAACCGCGAC
TCTTATGTATCTATGTTAGAGTTGTCAGAGATTTTTTATTTGCAACAGCAAATGCCAGCGGCGACTCAAATGTATGAACA
ATATGTGCGTACAGTGGGGCAAACCAATCAGGGTGCACGTGCGCTTTGGGTGGGGATTCGTGTTGCACGAGCCAATGGTG
ATCGAATGGGAATGCTGGCACTGATCAATCAACTGCGTGCTTTATTCCCAGATAGTTCAGAATATAAACGTTATTTGCAA
TTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_7)

(130-160)

Tetratricopeptide repeat (TPR_17)

(81-113)

Tetratricopeptide repeat (TPR_8)

(23-52)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

80.478

100

0.808
  pilF Acinetobacter baylyi ADP1

76.613

99.2

0.76