Detailed information NTG0063457

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: BTOYO_RS18975
• Genome accession: NC_022781
• Coordinates: 3713704..3717429 (+)
• Length: 1241 a.a.
• NCBI ID: WP_000572316.1
• Uniprot ID: A0AAJ1NZE3
• Organism: Bacillus toyonensis BCT-7112
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 3708704..3722429

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BTOYO_RS18960 (Btoyo_3775)	-	3708736..3709329 (+)	594	WP_000347508.1	TVP38/TMEM64 family protein	-
BTOYO_RS18965 (Btoyo_3776)	lepB	3709512..3710075 (+)	564	WP_000751914.1	signal peptidase I	-
BTOYO_RS18970 (Btoyo_3777)	addB	3710192..3713707 (+)	3516	WP_000058647.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
BTOYO_RS18975 (Btoyo_3778)	addA	3713704..3717429 (+)	3726	WP_000572316.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
BTOYO_RS18980 (Btoyo_3779)	-	3717442..3717729 (+)	288	WP_000179958.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
BTOYO_RS18985 (Btoyo_3780)	gerPF	3717818..3718033 (-)	216	WP_001141566.1	spore germination protein GerPF	-
BTOYO_RS18990 (Btoyo_3781)	-	3718076..3718462 (-)	387	WP_000902321.1	spore germination protein GerPE	-
BTOYO_RS18995 (Btoyo_3782)	gerPD	3718478..3718672 (-)	195	WP_001052804.1	spore germination protein GerPD	-
BTOYO_RS19000 (Btoyo_3783)	gerPC	3718679..3719293 (-)	615	WP_001070754.1	spore germination protein GerPC	-
BTOYO_RS19005 (Btoyo_3784)	gerPB	3719362..3719568 (-)	207	WP_001012514.1	spore germination protein GerPB	-
BTOYO_RS19010 (Btoyo_3785)	gerPA	3719583..3719804 (-)	222	WP_001111187.1	spore germination protein GerPA	-
BTOYO_RS19015 (Btoyo_3786)	-	3719904..3720080 (-)	177	WP_000462849.1	aspartyl-phosphate phosphatase Spo0E family protein	-
BTOYO_RS19020	-	3720327..3721225 (+)	899	Protein_3718	fumarylacetoacetate hydrolase family protein	-
BTOYO_RS19025 (Btoyo_3789)	-	3721261..3721542 (-)	282	WP_000926856.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142805.99 Da
• Isoelectric Point: 4.8167

>NTDB_id=63457 BTOYO_RS18975 WP_000572316.1 3713704..3717429(+) (addA) [Bacillus toyonensis BCT-7112]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERMIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAALLGMLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVKLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENDEVKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYLIGEDVAAELLCIQQTEEEVLEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIRKLRAPIKTRPRFMEKKGLTYAERGTAVHTVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGNSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNYVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=63457 BTOYO_RS18975 WP_000572316.1 3713704..3717429(+) (addA) [Bacillus toyonensis BCT-7112]
ATGATAGAGAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAATGGACG
TGACATTTTAGTCGCAGCAGCAGCTGGATCGGGAAAAACAGCAGTATTAGTTGAACGTATGATTAAAAAGATTATTAATG
AGGAAAATCCAGTCGATGTCGATCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTACTAATTGATGAGCCAGGATCTCAACATGTAAGAAAACAGCTGAGCCTATTAAATAAAGC
ATCTATTTCAACGATCCATTCATTTTGTTTACAAGTAATTAGAGGGTATTACTACATGCTGGATGTTGATCCTCGTTTTC
GTATTGCAAATCAAACAGAAAATGAGTTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGCTATACGAGTGACCGTAGTGATGATGACTTACAAAGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACCTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAACATATT
CGTAAAGCAACCGAACTCGCAATGCTTCCTGACGGCCCGGCGCCTCGCGTTGAAACCCTGCAAGCGGATGCAGCTTTACT
TGGAATGTTATCATCAGCAGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTTGTGAAGCTCGTAAAAGTCTTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGTGAACAAAGTGAAAATGATGAAGTGAAGCCATCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCAGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTACCGTTTCCGACTAGCAG
AACCAGGCTTATTCTTAGGAAAGTATAAACGCTTCACGCAAGAAGGATCGGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCTAATAGGTGAAGATGTAGCGGCTGAATTATTATGCA
TTCAGCAAACGGAAGAAGAAGTACTAGAGGGTGAAGAAGGTACGGAAGTCGAAAAGGCGCAACTGGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGAAAAACGGATAGTATGCGACCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCTATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCAG
TATATGCAGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCACTTGCAGCAGTACTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGGTCATTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAAGAACAAGAAC
TTCATGAAAAACTAGAATGGTTTTATAACTTACTACAAGGATGGCGTGAATTCGCGCGCCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTATGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
GCGTGTATTATATGACCGCGCAAGGCAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGGACGGCGAGAGCTCTTGGTGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAGGGACTTGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAGTTCATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCAATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCTTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTCGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCTGGAGCGTCTTGCTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTGCCATTACAAAGTGAACGAAAAGAAGAAGTGTACGATAGGTTAATGTGGAAGTACGGGTATGAGG
AAGCAACCTCTCATCGTGCGAAGCAATCCGTTACAGAAATAAAGAGAAATTATCAGTCTGAAGAAGGCAGCGATAACGCC
TTTATTAGAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGATTAACGTACGCAGAGCGTGG
GACAGCAGTCCATACCGTTATGCAACATGTAGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAACAAATTGCTG
GAATGGTAAATAAGGAATTGTTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTTCCATTTACGATGATGCTATCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAATAGCGGGGAATCGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAAGAAGATG
GTATTACGCTAATCGATTTCAAAACAGATACGATTGAAGGTAAATTCCCAGGCGGATTTGAACAAGCAAAACCAATTTTA
GAAGATCGCTATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATTATGTTGTGAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534