Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   N8A75_RS13810 Genome accession   NZ_CP104878
Coordinates   2532044..2532454 (-) Length   136 a.a.
NCBI ID   WP_129110531.1    Uniprot ID   -
Organism   Bacillus subtilis strain 4ZT     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2527044..2537454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A75_RS13780 (N8A75_13780) - 2527131..2527271 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  N8A75_RS13785 (N8A75_13785) yqeF 2527577..2528308 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  N8A75_RS13790 (N8A75_13790) cwlH 2528560..2529312 (-) 753 WP_015251672.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  N8A75_RS13795 (N8A75_13795) yqeD 2529499..2530125 (+) 627 WP_015251671.1 TVP38/TMEM64 family protein -
  N8A75_RS13800 (N8A75_13800) gnd 2530144..2531037 (-) 894 WP_015251670.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  N8A75_RS13805 (N8A75_13805) yqeB 2531289..2532011 (+) 723 WP_010886572.1 hypothetical protein -
  N8A75_RS13810 (N8A75_13810) nucA/comI 2532044..2532454 (-) 411 WP_129110531.1 sporulation-specific Dnase NucB Machinery gene
  N8A75_RS13815 (N8A75_13815) - 2532650..2533000 (+) 351 Protein_2676 sigma-70 family RNA polymerase sigma factor -
  N8A75_RS13820 (N8A75_13820) spoIVCA 2533028..2534488 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  N8A75_RS13825 (N8A75_13825) - 2534446..2534624 (-) 179 Protein_2678 hypothetical protein -
  N8A75_RS13830 (N8A75_13830) arsC 2534979..2535398 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  N8A75_RS13835 (N8A75_13835) acr3 2535410..2536450 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  N8A75_RS13840 (N8A75_13840) arsK 2536473..2536913 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  N8A75_RS13845 (N8A75_13845) arsR 2536972..2537289 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14939.96 Da        Isoelectric Point: 5.1853

>NTDB_id=634561 N8A75_RS13810 WP_129110531.1 2532044..2532454(-) (nucA/comI) [Bacillus subtilis strain 4ZT]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIKDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=634561 N8A75_RS13810 WP_129110531.1 2532044..2532454(-) (nucA/comI) [Bacillus subtilis strain 4ZT]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAAGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.478

84.559

0.537