Detailed information NTG0634527

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: N8A75_RS08565
• Genome accession: NZ_CP104878
• Coordinates: 1643196..1644185 (+)
• Length: 329 a.a.
• NCBI ID: WP_003232026.1
• Uniprot ID: P51835
• Organism: Bacillus subtilis strain 4ZT
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1638196..1649185

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N8A75_RS08550 (N8A75_08550)	acpP	1638393..1638626 (+)	234	WP_003154310.1	acyl carrier protein	-
N8A75_RS08555 (N8A75_08555)	rncS	1638766..1639515 (+)	750	WP_003232030.1	ribonuclease III	-
N8A75_RS08560 (N8A75_08560)	smc	1639616..1643176 (+)	3561	WP_014479770.1	chromosome segregation protein SMC	-
N8A75_RS08565 (N8A75_08565)	pilA	1643196..1644185 (+)	990	WP_003232026.1	signal recognition particle-docking protein FtsY	Machinery gene
N8A75_RS08570 (N8A75_08570)	sivC	1644221..1644706 (-)	486	WP_003245834.1	Ig-like domain-containing protein	-
N8A75_RS08575 (N8A75_08575)	srpM	1644884..1645216 (+)	333	WP_003238590.1	putative DNA-binding protein	-
N8A75_RS08580 (N8A75_08580)	ffh	1645230..1646570 (+)	1341	WP_014479771.1	signal recognition particle protein	-
N8A75_RS08585 (N8A75_08585)	rpsP	1646676..1646948 (+)	273	WP_003220815.1	30S ribosomal protein S16	-
N8A75_RS08590 (N8A75_08590)	khpA	1646948..1647193 (+)	246	WP_014476792.1	KH domain-containing protein	-
N8A75_RS08595 (N8A75_08595)	ylqD	1647315..1647701 (+)	387	WP_014476793.1	YlqD family protein	-
N8A75_RS08600 (N8A75_08600)	rimM	1647706..1648230 (+)	525	WP_003232013.1	ribosome maturation factor RimM	-
N8A75_RS08605 (N8A75_08605)	trmD	1648227..1648958 (+)	732	WP_003232011.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36342.52 Da
• Isoelectric Point: 5.0164

>NTDB_id=634527 N8A75_RS08565 WP_003232026.1 1643196..1644185(+) (pilA) [Bacillus subtilis strain 4ZT]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=634527 N8A75_RS08565 WP_003232026.1 1643196..1644185(+) (pilA) [Bacillus subtilis strain 4ZT]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAACTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCCCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAACTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCTGGAAGCGATCC
GGCGGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGTCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAGTTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAGGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGATCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

3D structure

• AlphaFold DB: P51835

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	43.709	91.793	0.401