Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   LSG23_RS18505 Genome accession   NZ_CP088973
Coordinates   3714274..3715419 (+) Length   381 a.a.
NCBI ID   WP_077722992.1    Uniprot ID   -
Organism   Bacillus velezensis strain CMML20-16     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3709274..3720419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSG23_RS18485 (LSG23_18405) - 3710171..3710599 (-) 429 WP_032869448.1 DUF1934 domain-containing protein -
  LSG23_RS18490 (LSG23_18410) - 3710753..3711265 (+) 513 Protein_3583 bacteriocin biosynthesis protein AlbG -
  LSG23_RS18495 (LSG23_18415) - 3711272..3712648 (-) 1377 WP_077722990.1 YncE family protein -
  LSG23_RS18500 (LSG23_18420) - 3712688..3714043 (-) 1356 WP_077722991.1 YncE family protein -
  LSG23_RS18505 (LSG23_18425) rapF 3714274..3715419 (+) 1146 WP_077722992.1 tetratricopeptide repeat protein Regulator
  LSG23_RS18510 (LSG23_18430) phrF 3715403..3715522 (+) 120 WP_069473622.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  LSG23_RS18515 (LSG23_18435) - 3715721..3715924 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  LSG23_RS18520 (LSG23_18440) - 3715938..3716141 (+) 204 WP_069473623.1 hypothetical protein -
  LSG23_RS18525 (LSG23_18450) - 3716548..3716829 (+) 282 WP_014306021.1 hypothetical protein -
  LSG23_RS18530 (LSG23_18455) - 3716873..3717385 (+) 513 WP_077722993.1 stage II sporulation protein M -
  LSG23_RS18535 (LSG23_18460) - 3717976..3718194 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  LSG23_RS18540 (LSG23_18465) speB 3718364..3719236 (-) 873 WP_003151054.1 agmatinase -
  LSG23_RS18545 (LSG23_18470) speE 3719296..3720126 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45541.70 Da        Isoelectric Point: 4.6057

>NTDB_id=634336 LSG23_RS18505 WP_077722992.1 3714274..3715419(+) (rapF) [Bacillus velezensis strain CMML20-16]
MASVLSSSAIGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQGNYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKAAAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=634336 LSG23_RS18505 WP_077722992.1 3714274..3715419(+) (rapF) [Bacillus velezensis strain CMML20-16]
GTGGCGAGTGTTTTATCGTCTTCAGCGATTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTATTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTGGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGGAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAATATGAATTTTTAGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAGAATTTCGAGAAGGCTGCTGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567