Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LSG23_RS01805 Genome accession   NZ_CP088973
Coordinates   364650..365087 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain CMML20-16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 359650..370087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSG23_RS01770 (LSG23_01770) - 360249..360377 (-) 129 Protein_308 hypothetical protein -
  LSG23_RS01775 (LSG23_01775) - 360522..361724 (+) 1203 WP_077721768.1 GTP-binding protein -
  LSG23_RS01780 (LSG23_01780) - 361789..362925 (+) 1137 WP_077721769.1 zinc-dependent alcohol dehydrogenase -
  LSG23_RS01785 (LSG23_01785) - 362940..363374 (+) 435 WP_021494203.1 RDD family protein -
  LSG23_RS01790 (LSG23_01790) - 363492..363767 (+) 276 WP_369719109.1 YckD family protein -
  LSG23_RS01795 (LSG23_01795) - 363981..364211 (+) 231 Protein_313 family 1 glycosylhydrolase -
  LSG23_RS01800 (LSG23_01800) nin/comJ 364230..364628 (-) 399 WP_077721770.1 competence protein ComJ Regulator
  LSG23_RS01805 (LSG23_01805) nucA/comI 364650..365087 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LSG23_RS01810 (LSG23_01810) hxlB 365448..366005 (-) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  LSG23_RS01815 (LSG23_01815) hxlA 366002..366637 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  LSG23_RS01820 (LSG23_01820) - 366869..367231 (+) 363 WP_016936795.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=634271 LSG23_RS01805 WP_003156588.1 364650..365087(-) (nucA/comI) [Bacillus velezensis strain CMML20-16]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=634271 LSG23_RS01805 WP_003156588.1 364650..365087(-) (nucA/comI) [Bacillus velezensis strain CMML20-16]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641