Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LRO84_RS16025 Genome accession   NZ_CP088955
Coordinates   3385909..3386709 (-) Length   266 a.a.
NCBI ID   WP_231761957.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain ARu19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3380909..3391709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO84_RS16005 (LRO84_16005) - 3381974..3382672 (-) 699 WP_004794712.1 YfgM family protein -
  LRO84_RS16010 (LRO84_16010) hisS 3382701..3383993 (-) 1293 WP_017388395.1 histidine--tRNA ligase -
  LRO84_RS16015 (LRO84_16015) ispG 3383990..3385105 (-) 1116 WP_004641112.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LRO84_RS16020 (LRO84_16020) - 3385127..3385918 (-) 792 WP_231761956.1 helix-turn-helix domain-containing protein -
  LRO84_RS16025 (LRO84_16025) pilF 3385909..3386709 (-) 801 WP_231761957.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LRO84_RS16030 (LRO84_16030) rlmN 3386724..3387959 (-) 1236 WP_005036936.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LRO84_RS16035 (LRO84_16035) ndk 3388083..3388514 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  LRO84_RS16040 (LRO84_16040) iscX 3388628..3388828 (-) 201 WP_003654869.1 Fe-S cluster assembly protein IscX -
  LRO84_RS16045 (LRO84_16045) - 3388928..3389479 (-) 552 WP_003654868.1 phosphatase PAP2 family protein -
  LRO84_RS16050 (LRO84_16050) - 3389504..3390793 (-) 1290 WP_231763416.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30106.27 Da        Isoelectric Point: 9.0417

>NTDB_id=634216 LRO84_RS16025 WP_231761957.1 3385909..3386709(-) (pilF) [Acinetobacter calcoaceticus strain ARu19]
MSTPKLKTVFCMGVAVALLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGVL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQSLLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=634216 LRO84_RS16025 WP_231761957.1 3385909..3386709(-) (pilF) [Acinetobacter calcoaceticus strain ARu19]
TTGAGTACTCCAAAGTTAAAAACGGTATTTTGTATGGGAGTTGCAGTTGCGCTTTTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCCGAAAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCAGGTGACTTGG
ATTCGGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGTGATGCAACGGCAAATATGATGATGGGTGTTTTA
CTACAGCAAGAAGGTAGTAAGCCGAACTTAGAAAAAGCTGAACATTACTTTAAACGAGCAATTTCATCTGAACCAGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGAACGCTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGCACAACATTAGGTTATGACCAACGCTATCAGGCGTTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAGCAATCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAATCAA
TTACGTGCCTTGTTCCCAGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-133)

Tetratricopeptide repeat (TPR_16)

(43-81)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

94.361

100

0.944
  pilF Acinetobacter baylyi ADP1

75.769

97.744

0.741