Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LRO55_RS16025 Genome accession   NZ_CP088954
Coordinates   3397834..3398634 (-) Length   266 a.a.
NCBI ID   WP_231764110.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain ACa13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3392834..3403634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO55_RS16005 (LRO55_16005) - 3393899..3394597 (-) 699 WP_004641116.1 YfgM family protein -
  LRO55_RS16010 (LRO55_16010) hisS 3394626..3395918 (-) 1293 WP_017388395.1 histidine--tRNA ligase -
  LRO55_RS16015 (LRO55_16015) ispG 3395915..3397030 (-) 1116 WP_004641112.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LRO55_RS16020 (LRO55_16020) - 3397052..3397843 (-) 792 WP_231764109.1 helix-turn-helix domain-containing protein -
  LRO55_RS16025 (LRO55_16025) pilF 3397834..3398634 (-) 801 WP_231764110.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LRO55_RS16030 (LRO55_16030) rlmN 3398649..3399884 (-) 1236 WP_231764111.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LRO55_RS16035 (LRO55_16035) ndk 3400008..3400439 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  LRO55_RS16040 (LRO55_16040) iscX 3400553..3400753 (-) 201 WP_003654869.1 Fe-S cluster assembly protein IscX -
  LRO55_RS16045 (LRO55_16045) - 3400853..3401404 (-) 552 WP_003654868.1 phosphatase PAP2 family protein -
  LRO55_RS16050 (LRO55_16050) - 3401429..3402718 (-) 1290 WP_231765328.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30078.21 Da        Isoelectric Point: 9.0417

>NTDB_id=634169 LRO55_RS16025 WP_231764110.1 3397834..3398634(-) (pilF) [Acinetobacter calcoaceticus strain ACa13]
MSTPKLKTVFCMGVAVALLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGVL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQSLLANRDSYISMLELAEIFYLQQQIPAATQMYEQYARTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=634169 LRO55_RS16025 WP_231764110.1 3397834..3398634(-) (pilF) [Acinetobacter calcoaceticus strain ACa13]
TTGAGTACTCCAAAGTTAAAAACGGTATTTTGTATGGGAGTTGCAGTTGCGCTTTTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAAAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCAGGTGACTTGG
ATTCGGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGTGATGCAACGGCAAATATGATGATGGGTGTTTTA
CTACAGCAAGAAGGTAGTAAGCCGAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCAGATAA
TGCTCAAGCACATAATAACTATGGTACTTATTTATACCAAATGGAACGCTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGCACAACATTAGGTTATGACCAACGCTATCAGGCGTTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAGCAATCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGCTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAA
TTACGTGCCTTGTTCCCAGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-133)

Tetratricopeptide repeat (TPR_16)

(43-81)

Tetratricopeptide repeat (TPR_16)

(149-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

75.385

97.744

0.737