Detailed information NTG0634166

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRO55_RS14205
• Genome accession: NZ_CP088954
• Coordinates: 3027262..3028380 (+)
• Length: 372 a.a.
• NCBI ID: WP_004641663.1
• Uniprot ID: A0A0B2UBU9
• Organism: Acinetobacter calcoaceticus strain ACa13
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3022262..3033380

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRO55_RS14195 (LRO55_14195)	-	3025386..3026078 (-)	693	WP_231763960.1	YggS family pyridoxal phosphate-dependent enzyme	-
LRO55_RS14200 (LRO55_14200)	pilT	3026205..3027242 (+)	1038	WP_003653239.1	type IV pilus twitching motility protein PilT	Machinery gene
LRO55_RS14205 (LRO55_14205)	pilU	3027262..3028380 (+)	1119	WP_004641663.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LRO55_RS14210 (LRO55_14210)	fur	3028450..3028887 (-)	438	WP_003653242.1	ferric iron uptake transcriptional regulator	-
LRO55_RS14215 (LRO55_14215)	-	3028999..3029397 (+)	399	WP_003653245.1	outer membrane protein assembly factor BamE	-
LRO55_RS14220 (LRO55_14220)	-	3029465..3029776 (-)	312	WP_132072003.1	RnfH family protein	-
LRO55_RS14225 (LRO55_14225)	-	3029776..3030843 (-)	1068	WP_198077856.1	hypothetical protein	-
LRO55_RS14230 (LRO55_14230)	-	3030995..3031450 (-)	456	WP_017388663.1	bacteriohemerythrin	-
LRO55_RS14235 (LRO55_14235)	-	3031702..3032550 (-)	849	WP_003653249.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41873.07 Da
• Isoelectric Point: 6.6400

>NTDB_id=634166 LRO55_RS14205 WP_004641663.1 3027262..3028380(+) (pilU) [Acinetobacter calcoaceticus strain ACa13]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGDVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=634166 LRO55_RS14205 WP_004641663.1 3027262..3028380(+) (pilU) [Acinetobacter calcoaceticus strain ACa13]
ATGGATTTTAATGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCGCCATC
TATGAAAATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGGGATGTTATTGGTCAACTTTTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACGGCC
CGCTTTCGTGTGAGCGCTTTTCAGCAGCGTGACATGCCAGGCATGGTGCTACGCCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCGCCCGTTCTTAAAGATTTATCAATGACTAAACGCGGCATCATTATTTTTGTTGGTGCAACAG
GTACTGGTAAATCGACGTCTTTGGCTTCAATGATCAGCTATCGCAATCACAATACTAAAGGTCATATTATTACGATTGAA
GACCCAATCGAGTTTATTCATGAACATGCGGGCTGTATCATTACTCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACTTTGCGACAAGCACCAGATGTTATCTTAATTGGTGAGATTCGATCTCGTGAAGTTATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTGGCTACGATGCACGCTAACAACGCCAACCAAGCGCTCGATCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTTTACATGGACTTATCGCTTAACTTAAAGGCCATGATTGCGCA
GCAACTTATTCCAACACCAGATGGGAATTCACGTCGTGCAGCCATTGAGATTTTGATTAACTCGCCATTAATTTCAGACT
ACATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGTTCGCGTGAACTGGGTATGCAAACCTTTGACCAA
GCATTGTTTGATCTCTATAAAGCAGGCCAAATTACCTATAAAGATGCATTAAAACATGCCGACTCACCGAACGATTTGCG
TTTGACCATTAAGCTTGCCGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTTGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A0B2UBU9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	67.655	99.731	0.675
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	97.849	0.398
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36