Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PALA4_RS19255 Genome accession   NZ_CP104866
Coordinates   4162321..4163676 (-) Length   451 a.a.
NCBI ID   WP_004365026.1    Uniprot ID   A0A0H2ZKT5
Organism   Pseudomonas aeruginosa strain PALA4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4157321..4168676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA4_RS19220 (PALA4_03795) thiS 4157555..4157755 (-) 201 WP_003110013.1 sulfur carrier protein ThiS -
  PALA4_RS19225 (PALA4_03796) - 4157858..4158235 (-) 378 WP_003084513.1 DUF423 domain-containing protein -
  PALA4_RS19230 (PALA4_03797) mtgA 4158272..4159003 (+) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PALA4_RS19235 (PALA4_03798) - 4159008..4159613 (-) 606 WP_003084511.1 hypothetical protein -
  PALA4_RS19240 (PALA4_03799) rpoH 4159678..4160532 (-) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  PALA4_RS19245 (PALA4_03800) ftsX 4160646..4161653 (-) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  PALA4_RS19250 (PALA4_03801) ftsE 4161653..4162324 (-) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  PALA4_RS19255 (PALA4_03802) pilA 4162321..4163676 (-) 1356 WP_004365026.1 signal recognition particle-docking protein FtsY Machinery gene
  PALA4_RS19260 (PALA4_03803) - 4163797..4165194 (+) 1398 WP_003084498.1 M16 family metallopeptidase -
  PALA4_RS19265 (PALA4_03804) - 4165187..4166674 (+) 1488 WP_003110404.1 M16 family metallopeptidase -
  PALA4_RS19270 (PALA4_03805) rsmD 4166674..4167270 (+) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PALA4_RS19275 (PALA4_03806) - 4167273..4167839 (+) 567 WP_003110405.1 hypothetical protein -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 47939.36 Da        Isoelectric Point: 4.5795

>NTDB_id=634002 PALA4_RS19255 WP_004365026.1 4162321..4163676(-) (pilA) [Pseudomonas aeruginosa strain PALA4]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPPAP
VAEAPLAGDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASI
GEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVD
VAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAV
QAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKL
DGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=634002 PALA4_RS19255 WP_004365026.1 4162321..4163676(-) (pilA) [Pseudomonas aeruginosa strain PALA4]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGCCGGCGCCG
GTTGCCGAAGCGCCGCTCGCCGGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCA
ACCCGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTC
CGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGGCTGTCCAAGACCAGCGCCAGCATC
GGCGAAGGCATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGATCTGCTCGACGACATCGAGACGCGCCTGCT
GACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAACTGGCCG
ACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGAC
GTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAA
GAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGG
TCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTG
CAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGA
GGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCG
GCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAACTG
GACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGA
GGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(246-445)

SRP54-type protein, helical bundle domain (SRP54_N)

(161-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZKT5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.344

96.009

0.455