Detailed information NTG0633934

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRK24_RS15270
• Genome accession: NZ_CP088919
• Coordinates: 3313812..3314978 (-)
• Length: 388 a.a.
• NCBI ID: WP_007507060.1
• Uniprot ID: -
• Organism: Rhodanobacter denitrificans strain DSM-24678
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3308812..3319978

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRK24_RS15255 (LRK24_15255)	-	3309966..3310658 (-)	693	WP_043291942.1	response regulator transcription factor	-
LRK24_RS15260 (LRK24_15260)	-	3310804..3313014 (+)	2211	WP_231381839.1	TonB-dependent siderophore receptor	-
LRK24_RS15265 (LRK24_15265)	-	3313368..3313745 (+)	378	WP_007507059.1	BON domain-containing protein	-
LRK24_RS15270 (LRK24_15270)	pilU	3313812..3314978 (-)	1167	WP_007507060.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LRK24_RS15275 (LRK24_15275)	pilT	3315132..3316169 (-)	1038	WP_007507061.1	type IV pilus twitching motility protein PilT	Machinery gene
LRK24_RS15280 (LRK24_15280)	proC	3316538..3317362 (+)	825	WP_007507062.1	pyrroline-5-carboxylate reductase	-
LRK24_RS15285 (LRK24_15285)	-	3317359..3317934 (+)	576	WP_007507063.1	YggT family protein	-
LRK24_RS15290 (LRK24_15290)	-	3318703..3319527 (-)	825	WP_007507064.1	ABC transporter substrate-binding protein	-
LRK24_RS15295 (LRK24_15295)	-	3319532..3319972 (-)	441	WP_007507065.1	secondary thiamine-phosphate synthase enzyme YjbQ	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43422.86 Da
• Isoelectric Point: 6.7735

>NTDB_id=633934 LRK24_RS15270 WP_007507060.1 3313812..3314978(-) (pilU) [Rhodanobacter denitrificans strain DSM-24678]
MSDFDFTSFLKLMVHKKASDLFITAGVAPSMKVQGRIVPITQSPLSVQQARDMVLNVMTPGQREEFEKTHECQFAISAQG
VGRFRVSCFYQRNCVGMVLRRIESKIPTIEELTLPPVIKQLAMTKRGIIIFVGATGSGKSTSLASMIGYRNQNSTGHIIT
IEDPIEYVHKHEGCIITQREVGIDTDSWDNALKNTLRQAPDVIMIGEIRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRILHFFPEDRRQQLLMDLSLNLKGIVAQQLIPTPDGKARRVAVEILLGTPLVQDYIRQGEIHKLKEVMKESTLLGMKTF
DQSLVELYHAGEISYEDALRYADSSNEVRLRIKLAQGGDAHTLSQGLEGVELEESRDAQNFSSNFLNR

Nucleotide

• Length: 1167 bp

>NTDB_id=633934 LRK24_RS15270 WP_007507060.1 3313812..3314978(-) (pilU) [Rhodanobacter denitrificans strain DSM-24678]
ATGAGCGATTTCGATTTCACCTCATTCCTGAAGCTGATGGTGCACAAGAAGGCCTCCGACTTGTTCATCACGGCGGGCGT
CGCCCCCTCGATGAAGGTGCAGGGCCGGATCGTGCCGATCACGCAGAGTCCGCTCAGCGTGCAGCAGGCGCGCGACATGG
TGCTCAACGTGATGACGCCGGGCCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCCAGTTCGCGATCTCCGCGCAGGGC
GTGGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACTGCGTCGGCATGGTGCTGCGCCGGATCGAGTCGAAGATCCC
CACCATCGAGGAGCTGACGCTGCCGCCGGTGATCAAGCAGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCTCGGGCAAATCGACCTCGCTGGCGTCGATGATCGGCTACCGCAACCAGAACTCGACCGGTCACATCATCACC
ATCGAGGACCCGATCGAGTACGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGACAACGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAAATCCGCACCCGCGAGGGCA
TGGACCACGCGATCGCCTTTGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCGAACAACGCCAACCAGGCGATG
GACCGCATCCTGCACTTCTTCCCCGAGGACCGCCGCCAGCAACTGCTGATGGACCTGTCGCTCAACCTGAAGGGCATCGT
GGCGCAGCAGCTGATCCCCACGCCCGACGGCAAGGCGCGCCGGGTCGCGGTGGAGATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCCAGGGCGAGATCCACAAGCTGAAGGAGGTGATGAAGGAGTCCACCCTGCTCGGCATGAAGACCTTC
GACCAGAGCCTGGTCGAGCTGTACCACGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGACAGCTCCAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCATACCCTGTCGCAAGGCCTGGAAGGCGTCGAGCTGGAGG
AAAGCCGCGACGCGCAGAACTTCAGCAGCAATTTCCTCAACCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	66.578	96.392	0.642
pilU	Acinetobacter baylyi ADP1	63.764	91.753	0.585
pilU	Vibrio cholerae strain A1552	52.809	91.753	0.485
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.129	95.876	0.394