Detailed information NTG0633602

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: N7218_RS20050
• Genome accession: NZ_CP104848
• Coordinates: 4035454..4036446 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain DFK.1
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 4030454..4041446

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N7218_RS20030 (N7218_20030)	ygbM	4031092..4031868 (+)	777	WP_001136914.1	HPr family phosphocarrier protein	-
N7218_RS20035 (N7218_20035)	ygbN	4031957..4033321 (+)	1365	WP_000104433.1	GntP family transporter	-
N7218_RS20040 (N7218_20040)	-	4033464..4034507 (-)	1044	WP_001161583.1	MBL fold metallo-hydrolase	-
N7218_RS20045 (N7218_20045)	-	4034732..4035283 (+)	552	WP_001179444.1	flavodoxin family protein	-
N7218_RS20050 (N7218_20050)	rpoS	4035454..4036446 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
N7218_RS20055 (N7218_20055)	nlpD	4036509..4037603 (-)	1095	WP_306278776.1	murein hydrolase activator NlpD	-
N7218_RS20060 (N7218_20060)	pcm	4037788..4038414 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
N7218_RS20065 (N7218_20065)	surE	4038408..4039169 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
N7218_RS20070 (N7218_20070)	truD	4039150..4040199 (-)	1050	WP_000568920.1	tRNA pseudouridine(13) synthase TruD	-
N7218_RS20075 (N7218_20075)	ispF	4040196..4040675 (-)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
N7218_RS20080 (N7218_20080)	ispD	4040675..4041385 (-)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=633602 N7218_RS20050 WP_000081550.1 4035454..4036446(-) (rpoS) [Escherichia coli strain DFK.1]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=633602 N7218_RS20050 WP_000081550.1 4035454..4036446(-) (rpoS) [Escherichia coli strain DFK.1]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCATTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715