Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LRS40_RS09610 Genome accession   NZ_CP088265
Coordinates   1712818..1714251 (-) Length   477 a.a.
NCBI ID   WP_142487257.1    Uniprot ID   -
Organism   Leclercia sp. G3L     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1707818..1719251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRS40_RS09580 (LRS40_09580) elbB 1709393..1710046 (+) 654 WP_231313600.1 isoprenoid biosynthesis glyoxalase ElbB -
  LRS40_RS09585 (LRS40_09585) mtgA 1710043..1710768 (+) 726 WP_370649949.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LRS40_RS09590 (LRS40_09590) npr 1710765..1711037 (-) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  LRS40_RS09595 (LRS40_09595) rapZ 1711034..1711888 (-) 855 WP_142487259.1 RNase adapter RapZ -
  LRS40_RS09600 (LRS40_09600) ptsN 1711934..1712425 (-) 492 WP_039031625.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LRS40_RS09605 (LRS40_09605) hpf 1712508..1712795 (-) 288 WP_142487258.1 ribosome hibernation promoting factor -
  LRS40_RS09610 (LRS40_09610) rpoN/rpoN1 1712818..1714251 (-) 1434 WP_142487257.1 RNA polymerase factor sigma-54 Machinery gene
  LRS40_RS09615 (LRS40_09615) lptB 1714299..1715024 (-) 726 WP_142487256.1 LPS export ABC transporter ATP-binding protein -
  LRS40_RS09620 (LRS40_09620) lptA 1715031..1715585 (-) 555 WP_231313601.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LRS40_RS09625 (LRS40_09625) lptC 1715554..1716129 (-) 576 WP_142487254.1 LPS export ABC transporter periplasmic protein LptC -
  LRS40_RS09630 (LRS40_09630) kdsC 1716126..1716692 (-) 567 WP_106994894.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LRS40_RS09635 (LRS40_09635) kdsD 1716707..1717693 (-) 987 WP_231313602.1 arabinose-5-phosphate isomerase KdsD -
  LRS40_RS09640 (LRS40_09640) - 1717707..1718684 (-) 978 WP_142487252.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54203.98 Da        Isoelectric Point: 4.3155

>NTDB_id=633577 LRS40_RS09610 WP_142487257.1 1712818..1714251(-) (rpoN/rpoN1) [Leclercia sp. G3L]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLDD
ILESIGDDEIDLEEIEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMRPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=633577 LRS40_RS09610 WP_142487257.1 1712818..1714251(-) (rpoN/rpoN1) [Leclercia sp. G3L]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCTATTCGTCTGTTGCA
GCTGTCAACGCTGGAACTTCAGCAGGAACTCCAGCAGGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ATGAGGTAGAAACTCAGCAAACCCAGGATAACGAAACTCTCGACAGCGTTGATGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTCGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGCACGCGCGCAGACTACCAGGATGA
TGAATTGCCGGTGTATCAGGGCGAAACTACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACGCCCTTTT
CCGATACCGATCGCGCCATCGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTGACCGTGTCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTCGAAGAGATCGAAGCGGTTCTGAAGCGCGTTCAGCGTTTTGATCC
GGTTGGCGTCGCGGCACGCGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTTGTTAAAGAGACGCCGTGGCTGG
AAGAGGCTCGCCTGATCGTCAGCGAGCATCTGGATCTGCTGGCTAACCACGACTTCCGCTCCCTGATGCGCGTGACGCGG
CTGAAGGAAGAGATCCTGAAAGAAGCGGTCAATCTGATTCAGTCGCTGGATCCGCGTCCGGGGCAGTCTATCCAGACCAG
CGAGCCCGAGTACGTCATTCCCGATGTACTGGTGCGCAAGCATAACGGCCTGTGGACCGTGGAGTTGAATTCCGACAGCA
TCCCTCGTCTGCAGATAAATCAGCAGTACGCCTCGATGTGCACCAGCGCGCGCAACGACTCGGATAACCAATATATCCGC
AGTAATCTTCAGGAAGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTGCGCGTCAGCCGCTGCAT
CGTGGAACAACAGCAGGCGTTCTTTGAACAAGGTGAAGAATTTATGAGGCCGATGGTGCTGGCCGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAACACCGAAGGCGGCGGCGAAGCTTCGTCGACGGCCATCCGGGCGCTGGTGAAAAAGTT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTTG
CACGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482