Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N7219_RS05295 Genome accession   NZ_CP104846
Coordinates   1074932..1075372 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain DFS.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1069932..1080372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7219_RS05270 (N7219_05270) coaE 1070422..1071042 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  N7219_RS05275 (N7219_05275) - 1071067..1071111 (+) 45 WP_120795372.1 protein YacM -
  N7219_RS05280 (N7219_05280) guaC 1071267..1072310 (+) 1044 WP_001217320.1 GMP reductase -
  N7219_RS05285 (N7219_05285) hofC 1072345..1073547 (-) 1203 WP_000157289.1 protein transport protein HofC -
  N7219_RS05290 (N7219_05290) gspE 1073537..1074922 (-) 1386 Protein_1043 type II secretion system protein GspE -
  N7219_RS05295 (N7219_05295) pilA 1074932..1075372 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  N7219_RS05300 (N7219_05300) nadC 1075576..1076469 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N7219_RS05305 (N7219_05305) ampD 1076557..1077108 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N7219_RS05310 (N7219_05310) ampE 1077105..1077959 (+) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  N7219_RS05315 (N7219_05315) aroP 1078002..1079372 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=633484 N7219_RS05295 WP_000360914.1 1074932..1075372(-) (pilA) [Escherichia coli strain DFS.1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=633484 N7219_RS05295 WP_000360914.1 1074932..1075372(-) (pilA) [Escherichia coli strain DFS.1]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363