Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N7J25_RS00835 Genome accession   NZ_CP104750
Coordinates   121439..122803 (+) Length   454 a.a.
NCBI ID   WP_001085195.1    Uniprot ID   Q8E287
Organism   Streptococcus agalactiae strain TAH 10/84 dMrvR     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 116439..127803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7J25_RS00810 (N7J25_00810) rpoE 117140..117724 (+) 585 WP_000418425.1 DNA-directed RNA polymerase subunit delta -
  N7J25_RS00815 (N7J25_00815) - 117997..119601 (+) 1605 WP_000170432.1 CTP synthase -
  N7J25_RS00820 (N7J25_00820) - 119710..120636 (+) 927 WP_000723852.1 alpha/beta hydrolase -
  N7J25_RS00830 (N7J25_00830) - 120831..121277 (+) 447 WP_000049182.1 dUTP diphosphatase -
  N7J25_RS00835 (N7J25_00835) radA 121439..122803 (+) 1365 WP_001085195.1 DNA repair protein RadA Machinery gene
  N7J25_RS00840 (N7J25_00840) - 122939..123436 (+) 498 WP_000214743.1 carbonic anhydrase -
  N7J25_RS00845 (N7J25_00845) - 123590..124909 (+) 1320 WP_000759711.1 FAD-containing oxidoreductase -
  N7J25_RS00850 (N7J25_00850) gltX 125118..126572 (+) 1455 WP_001284505.1 glutamate--tRNA ligase -
  N7J25_RS00855 (N7J25_00855) - 126612..127580 (-) 969 WP_000668256.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49562.84 Da        Isoelectric Point: 6.5148

>NTDB_id=632968 N7J25_RS00835 WP_001085195.1 121439..122803(+) (radA) [Streptococcus agalactiae strain TAH 10/84 dMrvR]
MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKNARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGEIGLTGEIR
RVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKAVFST

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=632968 N7J25_RS00835 WP_001085195.1 121439..122803(+) (radA) [Streptococcus agalactiae strain TAH 10/84 dMrvR]
GTGGCTAAGAAAAAATCAGTCTTCACCTGTCAAGAGTGTGGTTACCAATCACCTAAGTATTTGGGACGTTGTCCAAATTG
CTCAGCCTGGTCTTCATTTGTAGAAGAGGTTGAAGTTCAAGAAGTTAAAAATGCACGAGTTAGTTTGAACGGTGAAAAGT
CCCGACCGACGAAGTTAAAAGATGTTTCCTCAATTAACTATTCTAGAACTAAGACTGATATGGATGAATTTAACCGTGTT
CTAGGAGGCGGCGTTGTACCAGGAAGTTTGGTTCTGATTGGAGGAGACCCTGGGATAGGTAAATCTACTTTATTACTACA
AGTATCAACTCAGCTAGCTAATAAAGGTACAGTATTATATGTCTCAGGTGAGGAATCTGCAGAACAGATTAAGTTACGAA
GTGAGCGTCTAGGCGATATCGATAATGAATTTTACCTTTATGCCGAGACTAATATGCAAAGTATTCGGTCGGAGATTGAG
AAGATTAAACCAGATTTTCTTATTATTGATTCTATACAAACTATTATGAGTCCCGAAGTATCAAGTGTCCAAGGCTCTGT
TAGCCAAGTTCGCGAAGTGACCGCTGAGTTGATGCAGTTAGCTAAAACTAATAATATTGCTACTTTTATTGTAGGTCATG
TGACTAAGGAGGGAACATTAGCAGGCCCTCGAATGCTAGAACATATGGTGGATACGGTTCTTTATTTTGAAGGGGAGCGT
CATCACACGTTCCGTATTTTACGGGCAGTCAAAAACCGTTTTGGTTCGACCAATGAAATAGGTATCTTTGAAATGCAGTC
TGGTGGTCTAGTTGAAGTTTTGAATCCAAGTCAAGTTTTTCTTGAAGAACGTTTAGACGGTGCAACAGGATCTGCTATTG
TTGTTACTATGGAAGGAACAAGGCCTATTTTAGCAGAGGTTCAAGCTTTAGTGACTCCGACTGTTTTTGGAAATGCTAAG
CGTACAACAACAGGTCTTGACTTTAATCGTGTCAGTCTTATTATGGCAGTTCTAGAAAAACGTTGTGGTTTATTGCTTCA
AAATCAAGATGCCTATTTAAAATCAGCGGGTGGTGTTAAATTGGATGAACCAGCAATTGATTTAGCTGTTGCTGTAGCTA
TTGCTTCCAGTTACAAAGAAAAACCCACAAATCCACAAGAATCATTTATTGGAGAAATTGGACTCACTGGTGAGATTCGT
CGCGTGACACGTATTGAGCAACGTATTAATGAGGCTAGTAAATTGGGCTTTACTAAAATTTACGCACCAAAGAATTCTCT
AGCTGGGATTGAAATACCTAAAGGCATTGATGTTATTGGTGTGACAACTGTTAGTCAGGTCTTAAAAGCAGTATTTTCAA
CATAA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E287

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.079

99.78

0.879
  radA Streptococcus pneumoniae D39

88.079

99.78

0.879
  radA Streptococcus pneumoniae R6

88.079

99.78

0.879
  radA Streptococcus pneumoniae TIGR4

88.079

99.78

0.879
  radA Streptococcus mitis NCTC 12261

88.079

99.78

0.879
  radA Streptococcus mitis SK321

87.859

99.78

0.877
  radA/sms Bacillus subtilis subsp. subtilis str. 168

62.914

99.78

0.628