Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LRM90_RS14595 Genome accession   NZ_CP088134
Coordinates   3021542..3022522 (-) Length   326 a.a.
NCBI ID   WP_001540889.1    Uniprot ID   A0A5I8WZN6
Organism   Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4493     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3016542..3027522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRM90_RS14565 (LRM90_14565) endA 3017000..3017707 (+) 708 WP_000286133.1 deoxyribonuclease I -
  LRM90_RS14570 (LRM90_14570) rsmE 3017784..3018515 (+) 732 WP_011264384.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LRM90_RS14575 (LRM90_14575) gshB 3018535..3019482 (+) 948 WP_000593243.1 glutathione synthase -
  LRM90_RS14580 (LRM90_14580) - 3019698..3020261 (+) 564 WP_001053171.1 YqgE/AlgH family protein -
  LRM90_RS14585 (LRM90_14585) ruvX 3020261..3020677 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  LRM90_RS14590 (LRM90_14590) - 3020724..3021410 (-) 687 WP_000098341.1 IclR family transcriptional regulator -
  LRM90_RS14595 (LRM90_14595) pilT 3021542..3022522 (-) 981 WP_001540889.1 type IV pilus twitching motility protein PilT Machinery gene
  LRM90_RS14600 (LRM90_14600) - 3022540..3023244 (+) 705 WP_023235233.1 YggS family pyridoxal phosphate-dependent enzyme -
  LRM90_RS14605 (LRM90_14605) - 3023263..3023829 (+) 567 WP_001094848.1 YggT family protein -
  LRM90_RS14610 (LRM90_14610) yggU 3023826..3024116 (+) 291 WP_001277204.1 DUF167 family protein YggU -
  LRM90_RS14615 (LRM90_14615) - 3024124..3024717 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  LRM90_RS14620 (LRM90_14620) hemW 3024710..3025846 (+) 1137 WP_023235234.1 radical SAM family heme chaperone HemW -
  LRM90_RS14625 (LRM90_14625) - 3025938..3026945 (-) 1008 WP_000252192.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36154.36 Da        Isoelectric Point: 7.5116

>NTDB_id=632820 LRM90_RS14595 WP_001540889.1 3021542..3022522(-) (pilT) [Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4493]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTLPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=632820 LRM90_RS14595 WP_001540889.1 3021542..3022522(-) (pilT) [Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4493]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCTGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5I8WZN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362