Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5922_RS19775 Genome accession   NZ_CP104681
Coordinates   4006364..4006804 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 2022CK-00450     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4001364..4011804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5922_RS19755 (N5922_19750) aroP 4002362..4003735 (+) 1374 WP_023157668.1 aromatic amino acid transporter AroP -
  N5922_RS19760 (N5922_19755) ampE 4003778..4004632 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  N5922_RS19765 (N5922_19760) ampD 4004629..4005180 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5922_RS19770 (N5922_19765) nadC 4005268..4006161 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5922_RS19775 (N5922_19770) pilA 4006364..4006804 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  N5922_RS19780 (N5922_19775) pilB 4006814..4008199 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  N5922_RS19785 (N5922_19780) hofC 4008189..4009391 (+) 1203 WP_000157266.1 protein transport protein HofC -
  N5922_RS19790 (N5922_19785) guaC 4009426..4010469 (-) 1044 WP_001217338.1 GMP reductase -
  N5922_RS19795 (N5922_19790) - 4010625..4010669 (-) 45 WP_120795372.1 protein YacM -
  N5922_RS19800 (N5922_19795) coaE 4010694..4011314 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=632398 N5922_RS19775 WP_000360904.1 4006364..4006804(+) (pilA) [Escherichia coli strain 2022CK-00450]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=632398 N5922_RS19775 WP_000360904.1 4006364..4006804(+) (pilA) [Escherichia coli strain 2022CK-00450]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363