Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N5922_RS02910 Genome accession   NZ_CP104681
Coordinates   585575..587008 (-) Length   477 a.a.
NCBI ID   WP_089584580.1    Uniprot ID   -
Organism   Escherichia coli strain 2022CK-00450     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 580575..592008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5922_RS02875 (N5922_02870) elbB 581267..581920 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  N5922_RS02880 (N5922_02875) mtgA 581917..582645 (+) 729 WP_000047093.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N5922_RS02885 (N5922_02880) yrbL 582642..583274 (-) 633 WP_000620410.1 PhoP regulatory network protein YrbL -
  N5922_RS02890 (N5922_02885) npr 583487..583759 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  N5922_RS02895 (N5922_02890) rapZ 583756..584610 (-) 855 WP_000243741.1 RNase adapter RapZ -
  N5922_RS02900 (N5922_02895) ptsN 584656..585147 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N5922_RS02905 (N5922_02900) hpf 585265..585552 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  N5922_RS02910 (N5922_02905) rpoN/rpoN1 585575..587008 (-) 1434 WP_089584580.1 RNA polymerase factor sigma-54 Machinery gene
  N5922_RS02915 (N5922_02910) lptB 587056..587781 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  N5922_RS02920 (N5922_02915) lptA 587788..588345 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N5922_RS02925 (N5922_02920) lptC 588314..588889 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  N5922_RS02930 (N5922_02925) kdsC 588886..589452 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N5922_RS02935 (N5922_02930) kdsD 589473..590459 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  N5922_RS02940 (N5922_02935) yrbG 590473..591450 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53978.82 Da        Isoelectric Point: 4.3639

>NTDB_id=632348 N5922_RS02910 WP_089584580.1 585575..587008(-) (rpoN/rpoN1) [Escherichia coli strain 2022CK-00450]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELLQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=632348 N5922_RS02910 WP_089584580.1 585575..587008(-) (rpoN/rpoN1) [Escherichia coli strain 2022CK-00450]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACTGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465