Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5914_RS19925 Genome accession   NZ_CP104665
Coordinates   4131423..4131863 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain 2021CK-00607     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4126423..4136863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5914_RS19905 (N5914_19910) aroP 4127421..4128794 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N5914_RS19910 (N5914_19915) ampE 4128837..4129691 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  N5914_RS19915 (N5914_19920) ampD 4129688..4130239 (-) 552 WP_022645179.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5914_RS19920 (N5914_19925) nadC 4130327..4131220 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5914_RS19925 (N5914_19930) pilA 4131423..4131863 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  N5914_RS19930 (N5914_19935) pilB 4131873..4133258 (+) 1386 WP_022645178.1 type II secretion system protein GspE Machinery gene
  N5914_RS19935 (N5914_19940) hofC 4133248..4134450 (+) 1203 WP_022645177.1 protein transport protein HofC -
  N5914_RS19940 (N5914_19945) guaC 4134485..4135528 (-) 1044 WP_001217337.1 GMP reductase -
  N5914_RS19945 (N5914_19950) - 4135684..4135728 (-) 45 WP_120795372.1 protein YacM -
  N5914_RS19950 (N5914_19955) coaE 4135753..4136373 (+) 621 WP_022645176.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=632312 N5914_RS19925 WP_000360914.1 4131423..4131863(+) (pilA) [Escherichia coli strain 2021CK-00607]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=632312 N5914_RS19925 WP_000360914.1 4131423..4131863(+) (pilA) [Escherichia coli strain 2021CK-00607]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363