Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LQE85_RS03075 Genome accession   NZ_CP088000
Coordinates   655385..656515 (-) Length   376 a.a.
NCBI ID   WP_068849022.1    Uniprot ID   A0AAP5EFW1
Organism   Stenotrophomonas rhizophila strain Bl2-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 650385..661515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQE85_RS03060 (LQE85_03060) - 651589..653001 (-) 1413 WP_095364265.1 DEAD/DEAH box helicase -
  LQE85_RS03065 (LQE85_03065) - 653491..654480 (+) 990 WP_093532895.1 fumarylacetoacetate hydrolase family protein -
  LQE85_RS03070 (LQE85_03070) maiA 654477..655145 (+) 669 WP_068849020.1 maleylacetoacetate isomerase -
  LQE85_RS03075 (LQE85_03075) pilU 655385..656515 (-) 1131 WP_068849022.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQE85_RS03080 (LQE85_03080) - 656614..657015 (+) 402 WP_093532893.1 DUF4398 domain-containing protein -
  LQE85_RS03085 (LQE85_03085) - 657012..657908 (+) 897 WP_249833716.1 hypothetical protein -
  LQE85_RS03090 (LQE85_03090) - 658371..658595 (+) 225 WP_068849028.1 YdcH family protein -
  LQE85_RS03095 (LQE85_03095) - 658806..660176 (+) 1371 WP_249833717.1 pyridoxal-phosphate dependent enzyme -
  LQE85_RS03100 (LQE85_03100) - 660307..661488 (+) 1182 WP_249833718.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41472.75 Da        Isoelectric Point: 6.2367

>NTDB_id=632227 LQE85_RS03075 WP_068849022.1 655385..656515(-) (pilU) [Stenotrophomonas rhizophila strain Bl2-2]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDSETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRAVISQRLVKGQDGRRLPATEVLINTPMIRDLLRRGQVHEIKPAMEASLEEGMESFDQCL
FRMAKGGIIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYSAATPAAITHGF

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=632227 LQE85_RS03075 WP_068849022.1 655385..656515(-) (pilU) [Stenotrophomonas rhizophila strain Bl2-2]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGCGCCCCGGTGTA
CATCAAGATCGAAGGCAAGCTGTACCCGCTGGGCAATACCGGGCTGCCGCCCGGGATGGTCAAGAAAATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAACGCGACCTTGAGCTCAACATGGCCATCGCCCTGCCGGATGCGGGACGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGTGCCATCCGCAGCAAGATTCCCAGCATCGA
AGAGCTCAACCTGCCCCAGGTCCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAAGTCGGGCTGGATACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGATTCGGAAACGATGGAGGCGG
CCATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGTGCGCACCGCAACGTGCTGATGAACCTCTCGCTGAACCTGCGCGCGGTGATTTCCCAGCG
GCTGGTCAAGGGCCAGGATGGGCGGCGCCTGCCGGCCACCGAAGTGTTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGTCAGGTGCACGAGATCAAGCCGGCGATGGAAGCCTCGCTGGAAGAAGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGCATGGCCAAGGGCGGCATCATCGAGCAGGAAGAAGCGCTGCGTGCAGCCGATTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCGGCAGCGGCGAGCATGACCCGTACGCGGATTATTCGGCGGCGACCCCGGCGGCGATCACGC
ACGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

95.479

0.492
  pilU Acinetobacter baylyi ADP1

50

94.149

0.471
  pilU Vibrio cholerae strain A1552

48.333

95.745

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.717

100

0.444
  pilT Pseudomonas aeruginosa PAK

43.195

89.894

0.388
  pilT Acinetobacter baylyi ADP1

41.765

90.426

0.378
  pilT Acinetobacter baumannii strain A118

42.012

89.894

0.378
  pilT Acinetobacter nosocomialis M2

42.012

89.894

0.378
  pilT Acinetobacter baumannii D1279779

42.012

89.894

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.716

89.894

0.375
  pilT Legionella pneumophila strain Lp02

40.828

89.894

0.367
  pilT Legionella pneumophila strain ERS1305867

40.828

89.894

0.367
  pilT Neisseria meningitidis 8013

39.481

92.287

0.364
  pilT Neisseria gonorrhoeae MS11

39.481

92.287

0.364
  pilT Vibrio cholerae strain A1552

40.841

88.564

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.564

0.362