Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BBK_RS04915 Genome accession   NC_022659
Coordinates   1145223..1146542 (+) Length   439 a.a.
NCBI ID   WP_004526057.1    Uniprot ID   A0AAX0UF52
Organism   Burkholderia pseudomallei NCTC 13179     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1140223..1151542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBK_RS04885 (BBK_940) - 1140734..1141348 (+) 615 WP_004200150.1 50S ribosomal protein L25/general stress protein Ctc -
  BBK_RS04890 (BBK_941) pth 1141462..1142067 (+) 606 WP_009972861.1 aminoacyl-tRNA hydrolase -
  BBK_RS04895 (BBK_942) hisC 1142156..1143226 (+) 1071 WP_011204217.1 histidinol-phosphate transaminase -
  BBK_RS04900 (BBK_943) - 1143405..1143671 (-) 267 WP_004195247.1 YfhL family 4Fe-4S dicluster ferredoxin -
  BBK_RS04905 (BBK_944) coaD 1143734..1144234 (-) 501 WP_004195249.1 pantetheine-phosphate adenylyltransferase -
  BBK_RS04910 (BBK_945) rsmD 1144345..1144971 (-) 627 WP_004195251.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BBK_RS04915 (BBK_946) pilA 1145223..1146542 (+) 1320 WP_004526057.1 signal recognition particle-docking protein FtsY Machinery gene
  BBK_RS04920 (BBK_947) maiA 1146699..1147343 (-) 645 WP_009972858.1 maleylacetoacetate isomerase -
  BBK_RS32875 - 1147340..1147681 (-) 342 WP_009918580.1 hypothetical protein -
  BBK_RS04930 (BBK_948) nirB 1147728..1150172 (+) 2445 WP_004548109.1 nitrite reductase large subunit NirB -
  BBK_RS04935 (BBK_949) - 1150248..1150643 (+) 396 WP_009972856.1 nitrite reductase (NAD(P)H) small subunit -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 46370.66 Da        Isoelectric Point: 6.0726

>NTDB_id=63218 BBK_RS04915 WP_004526057.1 1145223..1146542(+) (pilA) [Burkholderia pseudomallei NCTC 13179]
MFSFFKRFKKAPDAAPAEPQQAPDAPRDASRAPASPDAARVDAPAAPQPRDERSENHDRPEHDETPTAEHSRSGAQPAAR
ATPPASAAASEAPVGQPRAAAARASEPAPSVVMTVTPSSNGRHGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSI
TNVFVNTKIDDALYEELETALLMSDAGIDATEHLLDALREKVRAGRLTDPQQVKDALRGLLVELLAPLEKSLVLGRAQPL
VMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARN
IDVMMADTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGG
ILAAIARQRPIPVYFIGIGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=63218 BBK_RS04915 WP_004526057.1 1145223..1146542(+) (pilA) [Burkholderia pseudomallei NCTC 13179]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAGCGCCCGATGCCGCCCCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCG
GGACGCTTCCCGCGCACCGGCATCGCCCGACGCCGCGCGAGTCGACGCGCCGGCCGCGCCGCAGCCGCGCGACGAGCGCA
GCGAAAACCACGATCGCCCCGAGCACGACGAAACGCCGACCGCCGAGCATTCCCGTTCCGGCGCGCAACCCGCCGCGCGC
GCGACGCCGCCCGCGTCCGCCGCCGCGTCGGAAGCGCCGGTCGGGCAGCCGCGCGCCGCCGCCGCGCGCGCAAGCGAGCC
CGCGCCGTCGGTCGTGATGACGGTCACGCCGTCGTCGAACGGCCGCCACGGCGTCGTCGAGACGGTCGAGATCGTGCCGC
CGCCCGCGCCCGAGCCGGCCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATC
ACGAACGTATTCGTCAACACGAAAATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGG
CATCGACGCGACCGAGCATCTGCTCGACGCGCTGCGCGAAAAGGTGCGCGCGGGCCGCCTCACCGATCCGCAGCAGGTGA
AGGACGCGCTGCGCGGGCTGCTCGTCGAGCTGCTGGCGCCGCTCGAGAAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTC
GTGATGATGATCGCGGGCGTGAACGGCGCCGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGA
TCAATCGGTGCTGCTCGCCGCGGGCGACACGTTTCGCGCCGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAACGCAACA
ACGTGACCGTCGTCCAGCAGGAAAGCGGCGACCCCGCCGCGGTGATCTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAAC
ATCGACGTGATGATGGCCGACACCGCAGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCG
CGTGATCGGCAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCG
CGCAGGTAAAGGCGTTCGACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGC
ATCCTCGCCGCGATCGCGCGCCAGCGGCCGATTCCGGTGTACTTCATCGGCATCGGCGAGAAGGTCGAGGATCTGCAGCC
GTTCAGCGCGGAGGAATTCGCGGACGCGCTGCTCGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(158-218)

SRP54-type protein, GTPase domain (SRP54)

(240-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.113

100

0.506

Multiple sequence alignment