Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOK81_RS10185 Genome accession   NZ_CP087992
Coordinates   2231305..2233038 (+) Length   577 a.a.
NCBI ID   WP_012338105.1    Uniprot ID   A0AAW6HRY9
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8433     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2226305..2238038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOK81_RS10165 (LOK81_10085) pilC 2226632..2227834 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  LOK81_RS10170 (LOK81_10090) - 2228275..2228724 (+) 450 WP_012338101.1 pilin -
  LOK81_RS10175 (LOK81_10095) - 2228966..2229193 (-) 228 WP_012338102.1 hypothetical protein -
  LOK81_RS10180 (LOK81_10100) - 2230026..2230466 (+) 441 WP_012338104.1 pilin -
  LOK81_RS10185 (LOK81_10105) pilB 2231305..2233038 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOK81_RS10190 (LOK81_10110) pilR 2233132..2234544 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator Regulator
  LOK81_RS10195 (LOK81_10115) - 2234731..2236341 (-) 1611 WP_004084601.1 ATP-binding protein -
  LOK81_RS10200 (LOK81_10120) sucC 2236596..2237759 (+) 1164 WP_004084603.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63061.17 Da        Isoelectric Point: 6.0946

>NTDB_id=632136 LOK81_RS10185 WP_012338105.1 2231305..2233038(+) (pilB) [Xylella fastidiosa subsp. multiplex strain CFBP8433]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALE
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFINKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLQGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPK
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=632136 LOK81_RS10185 WP_012338105.1 2231305..2233038(+) (pilB) [Xylella fastidiosa subsp. multiplex strain CFBP8433]
ATGAATACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TGAGTGCAGCAAAATTGGCCGCGGCCAATGCACTTGAGTTCGGGATGCCGCTGCTGGATGTGTCCGCATTCGACCACAAT
CACAATGCAATGAACTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGACGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAG
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACAGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCATCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTACTGGTCACTGGTCCGACCGGTTCGGGCAAGACCGTGTCACTGTA
CACTGCACTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCAGGGTG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTGATCATGGTCGGCGAAATCCGGGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACACTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGTTCAATTGCCGAAA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.319

98.267

0.563
  pilB Acinetobacter baumannii D1279779

56.79

98.267

0.558
  pilB Legionella pneumophila strain ERS1305867

51.232

98.44

0.504
  pilF Neisseria gonorrhoeae MS11

50.439

98.614

0.497
  pilB Vibrio cholerae strain A1552

50

94.281

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

46.749

98.614

0.461
  pilB Vibrio campbellii strain DS40M4

46.737

98.267

0.459