Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5855_RS16875 Genome accession   NZ_CP104629
Coordinates   3500724..3502220 (-) Length   498 a.a.
NCBI ID   WP_021557285.1    Uniprot ID   -
Organism   Escherichia coli strain AHM21C2872I     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3495724..3507220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5855_RS16850 (N5855_16810) panM 3495946..3496329 (+) 384 WP_040235041.1 aspartate 1-decarboxylase autocleavage activator PanM -
  N5855_RS16855 (N5855_16815) livJ 3496529..3497632 (-) 1104 WP_001626133.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  N5855_RS16860 (N5855_16820) rpoH 3497903..3498757 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  N5855_RS16865 (N5855_16825) ftsX 3499002..3500060 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  N5855_RS16870 (N5855_16830) ftsE 3500053..3500721 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  N5855_RS16875 (N5855_16835) pilA 3500724..3502220 (-) 1497 WP_021557285.1 signal recognition particle-docking protein FtsY Machinery gene
  N5855_RS16880 (N5855_16840) rsmD 3502370..3502966 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  N5855_RS16885 (N5855_16845) yhhL 3502956..3503225 (+) 270 WP_001295207.1 DUF1145 family protein -
  N5855_RS16890 (N5855_16850) yhhM 3503228..3503587 (-) 360 WP_000042887.1 DUF2500 domain-containing protein -
  N5855_RS16895 (N5855_16855) yhhN 3503728..3504354 (+) 627 WP_000964718.1 lysoplasmalogenase -
  N5855_RS16900 (N5855_16860) zntA 3504428..3506626 (+) 2199 WP_001626134.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  N5855_RS16905 (N5855_16865) tusA 3506728..3506973 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54611.45 Da        Isoelectric Point: 4.1579

>NTDB_id=631906 N5855_RS16875 WP_021557285.1 3500724..3502220(-) (pilA) [Escherichia coli strain AHM21C2872I]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEGVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=631906 N5855_RS16875 WP_021557285.1 3500724..3502220(-) (pilA) [Escherichia coli strain AHM21C2872I]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTAGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGGCGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGAT
GTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGC
CGTTTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCTTGATTGCCGATACCGCCGGACGTCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

44.543

90.161

0.402