Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5861_RS03240 Genome accession   NZ_CP104598
Coordinates   675057..675497 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain AHM8C160RI     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 670057..680497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5861_RS03215 (N5861_03210) coaE 670547..671167 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  N5861_RS03220 (N5861_03215) - 671192..671236 (+) 45 WP_120795372.1 protein YacM -
  N5861_RS03225 (N5861_03220) guaC 671392..672435 (+) 1044 WP_001217338.1 GMP reductase -
  N5861_RS03230 (N5861_03225) hofC 672470..673672 (-) 1203 WP_000157229.1 protein transport protein HofC -
  N5861_RS03235 (N5861_03230) pilB 673662..675047 (-) 1386 WP_250308222.1 type II secretion system protein GspE Machinery gene
  N5861_RS03240 (N5861_03235) pilA 675057..675497 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  N5861_RS03245 (N5861_03240) nadC 675700..676593 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5861_RS03250 (N5861_03245) ampD 676681..677232 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5861_RS03255 (N5861_03250) ampE 677229..678083 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  N5861_RS03260 (N5861_03255) aroP 678126..679499 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=631618 N5861_RS03240 WP_000360895.1 675057..675497(-) (pilA) [Escherichia coli strain AHM8C160RI]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=631618 N5861_RS03240 WP_000360895.1 675057..675497(-) (pilA) [Escherichia coli strain AHM8C160RI]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363