Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5B57_RS19680 Genome accession   NZ_CP104595
Coordinates   4045717..4046157 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain EC9952     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4040717..4051157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B57_RS19660 (N5B57_19610) aroP 4041715..4043088 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N5B57_RS19665 (N5B57_19615) ampE 4043131..4043985 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N5B57_RS19670 (N5B57_19620) ampD 4043982..4044533 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5B57_RS19675 (N5B57_19625) nadC 4044621..4045514 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5B57_RS19680 (N5B57_19630) pilA 4045717..4046157 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  N5B57_RS19685 (N5B57_19635) pilB 4046167..4047552 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  N5B57_RS19690 (N5B57_19640) hofC 4047542..4048744 (+) 1203 WP_000157234.1 protein transport protein HofC -
  N5B57_RS19695 (N5B57_19645) guaC 4048779..4049822 (-) 1044 WP_087889645.1 GMP reductase -
  N5B57_RS19700 (N5B57_19650) - 4049978..4050022 (-) 45 WP_120795372.1 protein YacM -
  N5B57_RS19705 (N5B57_19655) coaE 4050047..4050667 (+) 621 WP_001269531.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=631584 N5B57_RS19680 WP_000360904.1 4045717..4046157(+) (pilA) [Escherichia coli strain EC9952]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=631584 N5B57_RS19680 WP_000360904.1 4045717..4046157(+) (pilA) [Escherichia coli strain EC9952]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363