Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5B57_RS01335 Genome accession   NZ_CP104595
Coordinates   292101..293597 (+) Length   498 a.a.
NCBI ID   WP_001040652.1    Uniprot ID   -
Organism   Escherichia coli strain EC9952     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 287101..298597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B57_RS01305 (N5B57_01305) tusA 287348..287593 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  N5B57_RS01310 (N5B57_01310) zntA 287695..289893 (-) 2199 WP_063120062.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  N5B57_RS01315 (N5B57_01315) yhhN 289967..290593 (-) 627 WP_000964718.1 lysoplasmalogenase -
  N5B57_RS01320 (N5B57_01320) yhhM 290734..291093 (+) 360 WP_000042901.1 DUF2500 domain-containing protein -
  N5B57_RS01325 (N5B57_01325) yhhL 291096..291365 (-) 270 WP_001295207.1 DUF1145 family protein -
  N5B57_RS01330 (N5B57_01330) rsmD 291355..291951 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  N5B57_RS01335 (N5B57_01335) pilA 292101..293597 (+) 1497 WP_001040652.1 signal recognition particle-docking protein FtsY Machinery gene
  N5B57_RS01340 (N5B57_01340) ftsE 293600..294268 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  N5B57_RS01345 (N5B57_01345) ftsX 294261..295319 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  N5B57_RS01350 (N5B57_01350) rpoH 295564..296418 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  N5B57_RS01355 (N5B57_01355) livJ 296689..297792 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  N5B57_RS01360 (N5B57_01360) panM 297991..298374 (-) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54657.43 Da        Isoelectric Point: 4.1468

>NTDB_id=631530 N5B57_RS01335 WP_001040652.1 292101..293597(+) (pilA) [Escherichia coli strain EC9952]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVAEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=631530 N5B57_RS01335 WP_001040652.1 292101..293597(+) (pilA) [Escherichia coli strain EC9952]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCAGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCAGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTAGAAGAACAGTTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCTGCGAAGG
CGCGTAATGTTGACGTACTGATTGCCGATACAGCCGGACGCTTGCAGAACAAATCGCACCTGATGGAAGAGCTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTTCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)

SRP54-type protein, GTPase domain (SRP54)

(294-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.571

77.309

0.375