Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5B54_RS01400 Genome accession   NZ_CP104592
Coordinates   304600..306096 (+) Length   498 a.a.
NCBI ID   WP_001040681.1    Uniprot ID   -
Organism   Escherichia coli strain EC5931     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299600..311096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B54_RS01370 (N5B54_01365) tusA 299847..300092 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  N5B54_RS01375 (N5B54_01370) zntA 300194..302392 (-) 2199 WP_000106576.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  N5B54_RS01380 (N5B54_01375) yhhN 302466..303092 (-) 627 WP_000964718.1 lysoplasmalogenase -
  N5B54_RS01385 (N5B54_01380) yhhM 303233..303592 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  N5B54_RS01390 (N5B54_01385) yhhL 303595..303864 (-) 270 WP_001295207.1 DUF1145 family protein -
  N5B54_RS01395 (N5B54_01390) rsmD 303854..304450 (-) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  N5B54_RS01400 (N5B54_01395) pilA 304600..306096 (+) 1497 WP_001040681.1 signal recognition particle-docking protein FtsY Machinery gene
  N5B54_RS01405 (N5B54_01400) ftsE 306099..306767 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  N5B54_RS01410 (N5B54_01405) ftsX 306760..307818 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  N5B54_RS01415 (N5B54_01410) rpoH 308063..308917 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  N5B54_RS01420 (N5B54_01415) livJ 309188..310291 (+) 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  N5B54_RS01425 (N5B54_01420) ataR 310440..310706 (+) 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54672.49 Da        Isoelectric Point: 4.1449

>NTDB_id=631444 N5B54_RS01400 WP_001040681.1 304600..306096(+) (pilA) [Escherichia coli strain EC5931]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=631444 N5B54_RS01400 WP_001040681.1 304600..306096(+) (pilA) [Escherichia coli strain EC5931]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGAGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGATCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACAGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCTGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378