Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   EcoO8H8_RS13965 Genome accession   NZ_AP019856
Coordinates   2934366..2935358 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli O8:H8 strain 16F5M1D1     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 2929366..2940358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EcoO8H8_RS13940 (Eco16F5M1D1_2775) ygbN 2930017..2931381 (+) 1365 WP_000104439.1 GntP family transporter -
  EcoO8H8_RS13945 (Eco16F5M1D1_2776) - 2931420..2931656 (-) 237 WP_000562984.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  EcoO8H8_RS13950 (Eco16F5M1D1_2777) - 2931667..2933094 (-) 1428 WP_096307012.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  EcoO8H8_RS13955 (Eco16F5M1D1_2778) - 2933094..2933691 (-) 598 Protein_2762 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  EcoO8H8_RS13960 (Eco16F5M1D1_2780) - 2933838..2934245 (+) 408 WP_001208070.1 MarR family transcriptional regulator -
  EcoO8H8_RS13965 (Eco16F5M1D1_2781) rpoS 2934366..2935358 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  EcoO8H8_RS13970 (Eco16F5M1D1_2782) nlpD 2935421..2936515 (-) 1095 WP_349626797.1 murein hydrolase activator NlpD -
  EcoO8H8_RS13975 (Eco16F5M1D1_2783) pcm 2936700..2937326 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  EcoO8H8_RS13980 (Eco16F5M1D1_2784) surE 2937320..2938081 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  EcoO8H8_RS13985 (Eco16F5M1D1_2785) truD 2938062..2939111 (-) 1050 WP_000568924.1 tRNA pseudouridine(13) synthase TruD -
  EcoO8H8_RS13990 (Eco16F5M1D1_2786) ispF 2939108..2939587 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EcoO8H8_RS13995 (Eco16F5M1D1_2787) ispD 2939587..2940297 (-) 711 WP_000246136.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=63126 EcoO8H8_RS13965 WP_000081550.1 2934366..2935358(-) (rpoS) [Escherichia coli O8:H8 strain 16F5M1D1]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=63126 EcoO8H8_RS13965 WP_000081550.1 2934366..2935358(-) (rpoS) [Escherichia coli O8:H8 strain 16F5M1D1]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715

Multiple sequence alignment