Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5O72_RS18205 Genome accession   NZ_CP104536
Coordinates   3751875..3752315 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain E3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3746875..3757315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O72_RS18185 (N5O72_18185) aroP 3747873..3749246 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N5O72_RS18190 (N5O72_18190) ampE 3749289..3750143 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N5O72_RS18195 (N5O72_18195) ampD 3750140..3750691 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5O72_RS18200 (N5O72_18200) nadC 3750779..3751672 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5O72_RS18205 (N5O72_18205) pilA 3751875..3752315 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  N5O72_RS18210 (N5O72_18210) pilB 3752325..3753710 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  N5O72_RS18215 (N5O72_18215) hofC 3753700..3754902 (+) 1203 WP_000157227.1 protein transport protein HofC -
  N5O72_RS18220 (N5O72_18220) guaC 3754937..3755980 (-) 1044 WP_001217342.1 GMP reductase -
  N5O72_RS18225 (N5O72_18225) - 3756136..3756180 (-) 45 WP_120795372.1 protein YacM -
  N5O72_RS18230 (N5O72_18230) coaE 3756205..3756825 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=630613 N5O72_RS18205 WP_000360895.1 3751875..3752315(+) (pilA) [Escherichia coli strain E3]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=630613 N5O72_RS18205 WP_000360895.1 3751875..3752315(+) (pilA) [Escherichia coli strain E3]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363