Detailed information NTG0630385

Bioinformatically predicted

Overview

• Name: sepM
• Type: Regulator
• Locus tag: LO769_RS11175
• Genome accession: NZ_CP087600
• Coordinates: 2206699..2207727 (-)
• Length: 342 a.a.
• NCBI ID: WP_003132657.1
• Uniprot ID: S6FVM6
• Organism: Lactococcus lactis subsp. lactis strain IBB109
• Function: processing of CSP (predicted from homology) Competence regulation

Genomic Context

Location: 2201699..2212727

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LO769_RS11150 (LO769_11145)	-	2201985..2202482 (-)	498	WP_003132662.1	nitroreductase family protein	-
LO769_RS11155 (LO769_11150)	-	2202469..2202678 (-)	210	WP_003132661.1	hypothetical protein	-
LO769_RS11160 (LO769_11155)	-	2202675..2203502 (-)	828	WP_058221368.1	radical SAM protein	-
LO769_RS11165 (LO769_11160)	-	2203495..2205618 (-)	2124	WP_259743608.1	radical SAM protein	-
LO769_RS11170 (LO769_11165)	-	2205721..2206578 (-)	858	WP_003132658.1	Rgg/GadR/MutR family transcriptional regulator	-
LO769_RS11175 (LO769_11170)	sepM	2206699..2207727 (-)	1029	WP_003132657.1	SepM family pheromone-processing serine protease	Regulator
LO769_RS11180 (LO769_11175)	coaD	2207714..2208211 (-)	498	WP_010906348.1	pantetheine-phosphate adenylyltransferase	-
LO769_RS11185 (LO769_11180)	rsmD	2208259..2208798 (-)	540	WP_003132655.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
LO769_RS11190 (LO769_11185)	-	2208969..2210189 (-)	1221	WP_252172372.1	MFS transporter	-
LO769_RS11195 (LO769_11190)	lmrP	2210273..2211498 (-)	1226	Protein_2171	multidrug efflux MFS transporter LmrP	-
LO769_RS11200 (LO769_11195)	-	2211686..2212180 (+)	495	WP_012898649.1	RNA polymerase sigma factor	-

Sequence

Protein

• Length: 342 a.a.
• Molecular weight: 37349.96 Da
• Isoelectric Point: 9.9183

>NTDB_id=630385 LO769_RS11175 WP_003132657.1 2206699..2207727(-) (sepM) [Lactococcus lactis subsp. lactis strain IBB109]
MKKNKKINPKLKWGISIGLIVVALLALFYPTSYYVEMPGTTEPLGKMVKVEGKKDEHKGDFFLTTVQIARANLATMIYSH
FNSFTSIYSEQEMTGGLNDAQFNRVNQFYMETAQNTAIYQAFKLANKPYELKYEGVYVLDIAKNSTFKNKLELADTITAV
NGQQFTSSADMIAYVSKQKVGDSVTIEYTRIDGTKHKSTGKYIKIANGKTGIGISLVDHTEVVTTPKVTVNAGSIGGPSA
GMMFTLEIYSQLTGKDLRNGREIAGTGTIEHDGSIGQIGGVDKKVATASKEGAKVFLVPDSGTKKESSNNYLGAKAAAKK
LKTKMKIVPVKTIQDALDYLEK

Nucleotide

• Length: 1029 bp

>NTDB_id=630385 LO769_RS11175 WP_003132657.1 2206699..2207727(-) (sepM) [Lactococcus lactis subsp. lactis strain IBB109]
ATGAAAAAAAATAAAAAAATTAATCCCAAATTAAAATGGGGAATCTCGATTGGTCTAATTGTTGTTGCCCTTTTAGCTTT
ATTTTATCCAACAAGTTACTATGTGGAAATGCCCGGAACAACCGAGCCTTTAGGAAAAATGGTCAAAGTTGAAGGCAAAA
AAGATGAACATAAAGGTGATTTTTTCCTGACGACTGTCCAAATTGCACGAGCAAATCTTGCTACAATGATTTACAGCCAT
TTTAATAGTTTTACAAGCATTTACAGTGAGCAAGAAATGACTGGTGGACTTAACGACGCACAGTTCAACCGTGTCAACCA
GTTTTACATGGAAACGGCACAGAATACAGCGATTTATCAAGCTTTTAAATTAGCTAATAAACCTTATGAACTTAAATATG
AAGGCGTTTATGTCCTTGATATTGCCAAAAATTCGACCTTTAAAAATAAGCTAGAACTTGCAGATACGATTACAGCAGTC
AATGGTCAACAGTTTACATCAAGTGCCGACATGATTGCTTATGTTTCCAAACAAAAAGTCGGTGATTCAGTAACGATTGA
ATACACGAGAATTGACGGCACAAAGCACAAGTCAACTGGCAAATACATCAAAATTGCTAATGGAAAAACAGGAATTGGGA
TCAGCCTAGTTGACCATACTGAAGTCGTGACGACTCCAAAAGTTACTGTCAACGCGGGTTCAATCGGTGGTCCCTCTGCG
GGGATGATGTTTACACTAGAAATTTACAGTCAGTTGACAGGAAAAGATTTGCGAAACGGTCGCGAAATTGCTGGAACTGG
GACGATTGAACATGACGGAAGCATTGGCCAAATCGGCGGTGTTGATAAAAAAGTAGCCACAGCGAGCAAAGAAGGGGCAA
AGGTCTTCTTGGTGCCTGACTCAGGCACCAAAAAAGAAAGTAGCAACAATTACCTTGGAGCCAAAGCCGCCGCTAAAAAA
CTAAAAACGAAGATGAAAATCGTTCCCGTCAAAACTATTCAGGACGCTTTAGATTATTTAGAAAAATAA

Domains

Predicted by InterproScan.

• PDZ domain (PDZ_2) (134-194)

• Lon protease (S16) C-terminal proteolytic domain (Lon_C) (227-338)

3D structure

• AlphaFold DB: S6FVM6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
sepM	Streptococcus mutans UA159	48.696	100	0.491