Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   N5O74_RS25335 Genome accession   NZ_CP104509
Coordinates   5033381..5035210 (+) Length   609 a.a.
NCBI ID   WP_000035581.1    Uniprot ID   Q3YVE4
Organism   Escherichia coli strain E2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5028381..5040210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O74_RS25300 (N5O74_25300) ysgD 5028735..5028791 (+) 57 WP_211180520.1 protein YsgD -
  N5O74_RS25305 (N5O74_25305) corA 5028943..5029893 (+) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  N5O74_RS25310 (N5O74_25310) yigF 5029936..5030316 (-) 381 WP_000032581.1 DUF2628 domain-containing protein -
  N5O74_RS25315 (N5O74_25315) yigG 5030330..5030710 (-) 381 WP_000944218.1 protein YigG -
  N5O74_RS25320 (N5O74_25320) rarD 5030805..5031695 (-) 891 WP_000339104.1 EamA family transporter RarD -
  N5O74_RS25325 (N5O74_25325) yigI 5031747..5032214 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  N5O74_RS25330 (N5O74_25330) pldA 5032379..5033248 (+) 870 WP_001259700.1 phospholipase A -
  N5O74_RS25335 (N5O74_25335) recQ 5033381..5035210 (+) 1830 WP_000035581.1 ATP-dependent DNA helicase RecQ Machinery gene
  N5O74_RS25340 (N5O74_25340) rhtC 5035274..5035894 (+) 621 WP_000928824.1 threonine export protein RhtC -
  N5O74_RS25345 (N5O74_25345) rhtB 5035956..5036576 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  N5O74_RS25350 (N5O74_25350) pldB 5036687..5037709 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  N5O74_RS25355 (N5O74_25355) yigL 5037717..5038517 (+) 801 WP_000285362.1 sugar/pyridoxal phosphate phosphatase YigL -
  N5O74_RS25360 (N5O74_25360) bioP 5038593..5039492 (+) 900 WP_001196224.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68363.51 Da        Isoelectric Point: 7.2544

>NTDB_id=630134 N5O74_RS25335 WP_000035581.1 5033381..5035210(+) (recQ) [Escherichia coli strain E2]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=630134 N5O74_RS25335 WP_000035581.1 5033381..5035210(+) (recQ) [Escherichia coli strain E2]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCAGGCATT
ATCTACTGCAACAGCCGCGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCCGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391