Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5O81_RS19745 Genome accession   NZ_CP104503
Coordinates   4075931..4076371 (-) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli strain E1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4070931..4081371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O81_RS19720 (N5O81_19720) coaE 4071421..4072041 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  N5O81_RS19725 (N5O81_19725) - 4072066..4072110 (+) 45 WP_120795372.1 protein YacM -
  N5O81_RS19730 (N5O81_19730) guaC 4072266..4073309 (+) 1044 WP_001217343.1 GMP reductase -
  N5O81_RS19735 (N5O81_19735) hofC 4073344..4074546 (-) 1203 WP_000157292.1 protein transport protein HofC -
  N5O81_RS19740 (N5O81_19740) pilB 4074536..4075921 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  N5O81_RS19745 (N5O81_19745) pilA 4075931..4076371 (-) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  N5O81_RS19750 (N5O81_19750) nadC 4076575..4077468 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5O81_RS19755 (N5O81_19755) ampD 4077556..4078107 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5O81_RS19760 (N5O81_19760) ampE 4078104..4078958 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  N5O81_RS19765 (N5O81_19765) aroP 4079001..4080371 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=630042 N5O81_RS19745 WP_000360913.1 4075931..4076371(-) (pilA) [Escherichia coli strain E1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=630042 N5O81_RS19745 WP_000360913.1 4075931..4076371(-) (pilA) [Escherichia coli strain E1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363