Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5O81_RS13695 Genome accession   NZ_CP104503
Coordinates   2807973..2809469 (-) Length   498 a.a.
NCBI ID   WP_001040641.1    Uniprot ID   -
Organism   Escherichia coli strain E1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2802973..2814469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O81_RS13670 (N5O81_13670) panM 2803207..2803590 (+) 384 WP_000778808.1 aspartate 1-decarboxylase autocleavage activator PanM -
  N5O81_RS13675 (N5O81_13675) livJ 2803778..2804881 (-) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  N5O81_RS13680 (N5O81_13680) rpoH 2805152..2806006 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  N5O81_RS13685 (N5O81_13685) ftsX 2806251..2807309 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  N5O81_RS13690 (N5O81_13690) ftsE 2807302..2807970 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  N5O81_RS13695 (N5O81_13695) pilA 2807973..2809469 (-) 1497 WP_001040641.1 signal recognition particle-docking protein FtsY Machinery gene
  N5O81_RS13700 (N5O81_13700) rsmD 2809619..2810215 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  N5O81_RS13705 (N5O81_13705) yhhL 2810205..2810474 (+) 270 WP_001295207.1 DUF1145 family protein -
  N5O81_RS13710 (N5O81_13710) yhhM 2810477..2810836 (-) 360 WP_000042904.1 DUF2500 domain-containing protein -
  N5O81_RS13715 (N5O81_13715) yhhN 2810977..2811603 (+) 627 WP_000964718.1 lysoplasmalogenase -
  N5O81_RS13720 (N5O81_13720) zntA 2811677..2813875 (+) 2199 WP_000106604.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  N5O81_RS13725 (N5O81_13725) tusA 2814144..2814389 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54614.37 Da        Isoelectric Point: 4.1733

>NTDB_id=630011 N5O81_RS13695 WP_001040641.1 2807973..2809469(-) (pilA) [Escherichia coli strain E1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLSEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=630011 N5O81_RS13695 WP_001040641.1 2807973..2809469(-) (pilA) [Escherichia coli strain E1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378