Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5O75_RS20840 Genome accession   NZ_CP104500
Coordinates   4184757..4185197 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain E9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4179757..4190197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O75_RS20820 (N5O75_20825) aroP 4180755..4182128 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N5O75_RS20825 (N5O75_20830) ampE 4182171..4183025 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N5O75_RS20830 (N5O75_20835) ampD 4183022..4183573 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5O75_RS20835 (N5O75_20840) nadC 4183661..4184554 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5O75_RS20840 (N5O75_20845) pilA 4184757..4185197 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  N5O75_RS20845 (N5O75_20850) pilB 4185207..4186592 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  N5O75_RS20850 (N5O75_20855) hofC 4186582..4187784 (+) 1203 WP_000157234.1 protein transport protein HofC -
  N5O75_RS20855 (N5O75_20860) guaC 4187819..4188862 (-) 1044 WP_001217338.1 GMP reductase -
  N5O75_RS20860 (N5O75_20865) - 4189018..4189062 (-) 45 WP_120795372.1 protein YacM -
  N5O75_RS20865 (N5O75_20870) coaE 4189087..4189707 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=629945 N5O75_RS20840 WP_000360904.1 4184757..4185197(+) (pilA) [Escherichia coli strain E9]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=629945 N5O75_RS20840 WP_000360904.1 4184757..4185197(+) (pilA) [Escherichia coli strain E9]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363