Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5O75_RS01570 Genome accession   NZ_CP104500
Coordinates   335859..337352 (+) Length   497 a.a.
NCBI ID   WP_001040630.1    Uniprot ID   A7ZT00
Organism   Escherichia coli strain E9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330859..342352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O75_RS01540 (N5O75_01540) tusA 331106..331351 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  N5O75_RS01545 (N5O75_01545) zntA 331453..333651 (-) 2199 WP_038977221.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  N5O75_RS01550 (N5O75_01550) yhhN 333725..334351 (-) 627 WP_000964718.1 lysoplasmalogenase -
  N5O75_RS01555 (N5O75_01555) yhhM 334492..334851 (+) 360 WP_000042900.1 DUF2500 domain-containing protein -
  N5O75_RS01560 (N5O75_01560) yhhL 334854..335123 (-) 270 WP_001295207.1 DUF1145 family protein -
  N5O75_RS01565 (N5O75_01565) rsmD 335113..335709 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  N5O75_RS01570 (N5O75_01570) pilA 335859..337352 (+) 1494 WP_001040630.1 signal recognition particle-docking protein FtsY Machinery gene
  N5O75_RS01575 (N5O75_01575) ftsE 337355..338023 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  N5O75_RS01580 (N5O75_01580) ftsX 338016..339074 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  N5O75_RS01585 (N5O75_01585) rpoH 339319..340173 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  N5O75_RS01590 (N5O75_01590) livJ 340444..341547 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  N5O75_RS01595 (N5O75_01595) panM 341746..342129 (-) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54530.33 Da        Isoelectric Point: 4.1560

>NTDB_id=629892 N5O75_RS01570 WP_001040630.1 335859..337352(+) (pilA) [Escherichia coli strain E9]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETAEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=629892 N5O75_RS01570 WP_001040630.1 335859..337352(+) (pilA) [Escherichia coli strain E9]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAACCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGGCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGTAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGATGTG
GGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGT
TCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGTAA
CAATATCCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCGGTTATCTTCGACGCCATTCAGGCTGCGAAGGCGC
GTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.654

81.489

0.388