Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N4S17_RS12150 Genome accession   NZ_CP104472
Coordinates   2483654..2485150 (-) Length   498 a.a.
NCBI ID   WP_001040652.1    Uniprot ID   -
Organism   Escherichia coli strain UC4224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2478654..2490150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4S17_RS12125 (N4S17_12125) panM 2478878..2479261 (+) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
  N4S17_RS12130 (N4S17_12130) livJ 2479460..2480563 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  N4S17_RS12135 (N4S17_12135) rpoH 2480834..2481688 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  N4S17_RS12140 (N4S17_12140) ftsX 2481932..2482990 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  N4S17_RS12145 (N4S17_12145) ftsE 2482983..2483651 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  N4S17_RS12150 (N4S17_12150) pilA 2483654..2485150 (-) 1497 WP_001040652.1 signal recognition particle-docking protein FtsY Machinery gene
  N4S17_RS12155 (N4S17_12155) rsmD 2485300..2485896 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  N4S17_RS12160 (N4S17_12160) yhhL 2485886..2486155 (+) 270 WP_001295207.1 DUF1145 family protein -
  N4S17_RS12165 (N4S17_12165) yhhM 2486158..2486517 (-) 360 WP_000042900.1 DUF2500 domain-containing protein -
  N4S17_RS12170 (N4S17_12170) yhhN 2486658..2487284 (+) 627 WP_000964718.1 lysoplasmalogenase -
  N4S17_RS12175 (N4S17_12175) zntA 2487358..2489556 (+) 2199 WP_000106507.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  N4S17_RS12180 (N4S17_12180) tusA 2489658..2489903 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54657.43 Da        Isoelectric Point: 4.1468

>NTDB_id=629489 N4S17_RS12150 WP_001040652.1 2483654..2485150(-) (pilA) [Escherichia coli strain UC4224]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVAEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=629489 N4S17_RS12150 WP_001040652.1 2483654..2485150(-) (pilA) [Escherichia coli strain UC4224]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCAGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCAGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTAGAAGAACAGTTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
TAACAATATCCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCGGTTATCTTCGACGCCATTCAGGCTGCGAAGG
CGCGTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)

SRP54-type protein, GTPase domain (SRP54)

(294-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.571

77.309

0.375