Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LOC22_RS08110 Genome accession   NZ_CP087390
Coordinates   1683185..1684618 (+) Length   477 a.a.
NCBI ID   WP_023295498.1    Uniprot ID   -
Organism   Enterobacter sp. AN-K1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1678185..1689618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOC22_RS08080 (LOC22_08080) - 1678752..1679729 (+) 978 WP_126340367.1 calcium/sodium antiporter -
  LOC22_RS08085 (LOC22_08085) kdsD 1679743..1680729 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  LOC22_RS08090 (LOC22_08090) kdsC 1680744..1681310 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LOC22_RS08095 (LOC22_08095) lptC 1681307..1681882 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  LOC22_RS08100 (LOC22_08100) lptA 1681851..1682405 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LOC22_RS08105 (LOC22_08105) lptB 1682412..1683137 (+) 726 WP_023304493.1 LPS export ABC transporter ATP-binding protein -
  LOC22_RS08110 (LOC22_08110) rpoN/rpoN1 1683185..1684618 (+) 1434 WP_023295498.1 RNA polymerase factor sigma-54 Machinery gene
  LOC22_RS08115 (LOC22_08115) hpf 1684641..1684928 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  LOC22_RS08120 (LOC22_08120) ptsN 1685011..1685502 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LOC22_RS08125 (LOC22_08125) rapZ 1685548..1686402 (+) 855 WP_003861870.1 RNase adapter RapZ -
  LOC22_RS08130 (LOC22_08130) npr 1686399..1686671 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  LOC22_RS08135 (LOC22_08135) mtgA 1686711..1687436 (-) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LOC22_RS08140 (LOC22_08140) elbB 1687433..1688086 (-) 654 WP_032655241.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54098.98 Da        Isoelectric Point: 4.3549

>NTDB_id=629224 LOC22_RS08110 WP_023295498.1 1683185..1684618(+) (rpoN/rpoN1) [Enterobacter sp. AN-K1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGNDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=629224 LOC22_RS08110 WP_023295498.1 1683185..1684618(+) (rpoN/rpoN1) [Enterobacter sp. AN-K1]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTTGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGACGAAATCTACACCGCGGGTACCCCTTCCGGGACACGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCAATGACGAGATTGAACTGGAAGAGATTGAAGCCGTCCTGAAACGCGTTCAGCGCTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAACTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCATGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
TGAACCGGAATATGTCATCCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAATGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAGGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48