Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N4502_RS18755 Genome accession   NZ_CP104371
Coordinates   3892943..3893383 (-) Length   146 a.a.
NCBI ID   WP_000360907.1    Uniprot ID   -
Organism   Escherichia coli strain 2021CK-01784     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3887943..3898383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4502_RS18730 (N4502_18730) coaE 3888433..3889053 (-) 621 WP_001269513.1 dephospho-CoA kinase -
  N4502_RS18735 (N4502_18735) - 3889078..3889122 (+) 45 WP_120795372.1 protein YacM -
  N4502_RS18740 (N4502_18740) guaC 3889278..3890321 (+) 1044 WP_001217337.1 GMP reductase -
  N4502_RS18745 (N4502_18745) hofC 3890356..3891558 (-) 1203 WP_000157288.1 protein transport protein HofC -
  N4502_RS18750 (N4502_18750) pilB 3891548..3892933 (-) 1386 WP_001025168.1 type II secretion system protein GspE Machinery gene
  N4502_RS18755 (N4502_18755) pilA 3892943..3893383 (-) 441 WP_000360907.1 prepilin peptidase-dependent pilin Machinery gene
  N4502_RS18760 (N4502_18760) nadC 3893586..3894479 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N4502_RS18765 (N4502_18765) ampD 3894567..3895118 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N4502_RS18770 (N4502_18770) ampE 3895115..3895969 (+) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  N4502_RS18775 (N4502_18775) aroP 3896012..3897385 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15651.78 Da        Isoelectric Point: 4.3938

>NTDB_id=629095 N4502_RS18755 WP_000360907.1 3892943..3893383(-) (pilA) [Escherichia coli strain 2021CK-01784]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSTMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=629095 N4502_RS18755 WP_000360907.1 3892943..3893383(-) (pilA) [Escherichia coli strain 2021CK-01784]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGTGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACTACCCGC
TATGTTTCAACCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363