Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N4502_RS11510 Genome accession   NZ_CP104371
Coordinates   2364012..2365445 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 2021CK-01784     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2359012..2370445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4502_RS11480 (N4502_11480) yrbG 2359570..2360547 (+) 978 WP_000922873.1 calcium/sodium antiporter -
  N4502_RS11485 (N4502_11485) kdsD 2360561..2361547 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  N4502_RS11490 (N4502_11490) kdsC 2361568..2362134 (+) 567 WP_260837374.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N4502_RS11495 (N4502_11495) lptC 2362131..2362706 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  N4502_RS11500 (N4502_11500) lptA 2362675..2363232 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N4502_RS11505 (N4502_11505) lptB 2363239..2363964 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  N4502_RS11510 (N4502_11510) rpoN/rpoN1 2364012..2365445 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  N4502_RS11515 (N4502_11515) hpf 2365468..2365755 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  N4502_RS11520 (N4502_11520) ptsN 2365873..2366364 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N4502_RS11525 (N4502_11525) rapZ 2366410..2367264 (+) 855 WP_000243741.1 RNase adapter RapZ -
  N4502_RS11530 (N4502_11530) npr 2367261..2367533 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  N4502_RS11535 (N4502_11535) yrbL 2367746..2368378 (+) 633 WP_000620420.1 PhoP regulatory network protein YrbL -
  N4502_RS11540 (N4502_11540) mtgA 2368417..2369103 (-) 687 WP_000047062.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N4502_RS11545 (N4502_11545) elbB 2369100..2369753 (-) 654 WP_001306027.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=629062 N4502_RS11510 WP_000809051.1 2364012..2365445(+) (rpoN/rpoN1) [Escherichia coli strain 2021CK-01784]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=629062 N4502_RS11510 WP_000809051.1 2364012..2365445(+) (rpoN/rpoN1) [Escherichia coli strain 2021CK-01784]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGG
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTAGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465