Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LO008_RS00080 Genome accession   NZ_CP087351
Coordinates   18295..19095 (+) Length   266 a.a.
NCBI ID   WP_005136899.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain DB003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 13295..24095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LO008_RS00055 - 14205..15503 (+) 1299 WP_001991191.1 glycosyltransferase family 1 protein -
  LO008_RS00060 - 15528..16079 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  LO008_RS00065 iscX 16179..16379 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  LO008_RS00070 ndk 16493..16924 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  LO008_RS00075 rlmN 17047..18279 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LO008_RS00080 pilF 18295..19095 (+) 801 WP_005136899.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LO008_RS00085 - 19086..19877 (+) 792 WP_000405163.1 RodZ domain-containing protein -
  LO008_RS00090 ispG 19899..21014 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LO008_RS00095 hisS 21011..22303 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  LO008_RS00100 - 22332..23030 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30083.42 Da        Isoelectric Point: 9.3928

>NTDB_id=629045 LO008_RS00080 WP_005136899.1 18295..19095(+) (pilF) [Acinetobacter baumannii strain DB003]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGNI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=629045 LO008_RS00080 WP_005136899.1 18295..19095(+) (pilF) [Acinetobacter baumannii strain DB003]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCAATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722