Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LO011_RS02620 Genome accession   NZ_CP087340
Coordinates   552676..553476 (+) Length   266 a.a.
NCBI ID   WP_258579594.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain DB008     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 547676..558476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LO011_RS02595 - 548586..549884 (+) 1299 WP_085940557.1 glycosyltransferase family 1 protein -
  LO011_RS02600 - 549909..550460 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  LO011_RS02605 iscX 550560..550760 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  LO011_RS02610 ndk 550874..551305 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  LO011_RS02615 rlmN 551428..552660 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LO011_RS02620 pilF 552676..553476 (+) 801 WP_258579594.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LO011_RS02625 - 553467..554258 (+) 792 WP_258579596.1 helix-turn-helix domain-containing protein -
  LO011_RS02630 ispG 554280..555395 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LO011_RS02635 hisS 555392..556684 (+) 1293 WP_114155370.1 histidine--tRNA ligase -
  LO011_RS02640 - 556713..557411 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30093.46 Da        Isoelectric Point: 9.3928

>NTDB_id=628921 LO011_RS02620 WP_258579594.1 552676..553476(+) (pilF) [Acinetobacter baumannii strain DB008]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGNI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=628921 LO011_RS02620 WP_258579594.1 552676..553476(+) (pilF) [Acinetobacter baumannii strain DB008]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAACGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCAATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726