Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   N4267_RS01085 Genome accession   NZ_CP104354
Coordinates   230684..232888 (+) Length   734 a.a.
NCBI ID   WP_134986621.1    Uniprot ID   -
Organism   Vibrio cholerae strain PNUSAV001140     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 225684..237888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4267_RS01065 (N4267_01065) - 226763..227929 (-) 1167 WP_134986623.1 O-succinylhomoserine (thiol)-lyase -
  N4267_RS01070 (N4267_01070) metJ 228197..228514 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  N4267_RS01075 (N4267_01075) - 228626..229903 (-) 1278 WP_001278588.1 malic enzyme-like NAD(P)-binding protein -
  N4267_RS01080 (N4267_01080) rpmE 230211..230429 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  N4267_RS01085 (N4267_01085) priA 230684..232888 (+) 2205 WP_134986621.1 primosomal protein N' Machinery gene
  N4267_RS01090 (N4267_01090) cytR 233194..234201 (+) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  N4267_RS01095 (N4267_01095) - 234373..234924 (+) 552 WP_001884022.1 SPOR domain-containing protein -
  N4267_RS01100 (N4267_01100) hslV 235061..235618 (+) 558 WP_000208247.1 ATP-dependent protease subunit HslV -
  N4267_RS01105 (N4267_01105) hslU 235684..237015 (+) 1332 WP_001293366.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82064.36 Da        Isoelectric Point: 8.4848

>NTDB_id=628861 N4267_RS01085 WP_134986621.1 230684..232888(+) (priA) [Vibrio cholerae strain PNUSAV001140]
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPKLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PILARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVIHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALTGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEGQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLSS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=628861 N4267_RS01085 WP_134986621.1 230684..232888(+) (priA) [Vibrio cholerae strain PNUSAV001140]
ATGCGACCCTCCATTGCCCGAGTTTGCCTACCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGTGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGCCAGTTTTATCATTATCCGCTCGGTGAAACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGAAAAGCGGCAGAGCTGACTAGCCATAAAGAGTGGCGTTTGACCGCACTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCGGTAAAACAAGCGCAAGTTCTCCATTTGTTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGCACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAAGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGCTCAGGCAAAACAGAGGTGTATCTTAACCTCATCACC
CCTATATTGGCGCGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACCCCACAAACCATCAACCGGTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTGATACACTCGGCGCTCAACGATACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACACGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGCCTGCGTTATCACGCCCGAGATGTGGCTGTGATGCGCGCGCA
TCTGGAAAACATTCCGATCGTGCTTGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCACTGGTAAATACC
ATCACTTGCAACTGACGCAGCGCGCCGGTAATGCACTACCCACACGTAACCACGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGTCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGTCACGAGTGTGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAGATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCAACCCAGTTAGTGACGGTTGGGGTGGGGACGGAGCAACTGGAAGGGCAATTACACACTCTTTTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTCGCGTTGTTGGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGTTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGCGAAGTGGTGTTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGTAAGCTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAACTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGTCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

Helicase conserved C-terminal domain (Helicase_C)

(516-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

DEAD/DEAH box helicase (DEAD)

(205-364)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.572

100

0.439