Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LO004_RS17255 Genome accession   NZ_CP087331
Coordinates   3602422..3603222 (-) Length   266 a.a.
NCBI ID   WP_199984560.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain LMG994     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3597422..3608222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LO004_RS17235 - 3598487..3599185 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  LO004_RS17240 hisS 3599214..3600506 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  LO004_RS17245 ispG 3600503..3601618 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LO004_RS17250 - 3601640..3602431 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  LO004_RS17255 pilF 3602422..3603222 (-) 801 WP_199984560.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LO004_RS17260 rlmN 3603238..3604470 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LO004_RS17265 ndk 3604593..3605024 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  LO004_RS17270 iscX 3605138..3605338 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  LO004_RS17275 - 3605438..3605989 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  LO004_RS17280 - 3606014..3607312 (-) 1299 WP_001991191.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30103.45 Da        Isoelectric Point: 9.2310

>NTDB_id=628846 LO004_RS17255 WP_199984560.1 3602422..3603222(-) (pilF) [Acinetobacter baumannii strain LMG994]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNH
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=628846 LO004_RS17255 WP_199984560.1 3602422..3603222(-) (pilF) [Acinetobacter baumannii strain LMG994]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAT
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722