Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   N0470_RS21960 Genome accession   NZ_CP104326
Coordinates   4519274..4521103 (-) Length   609 a.a.
NCBI ID   WP_000035581.1    Uniprot ID   Q3YVE4
Organism   Escherichia coli strain THB42-F1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4514274..4526103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0470_RS21935 (N0470_21930) bioP 4514992..4515891 (-) 900 WP_001196238.1 biotin transporter -
  N0470_RS21940 (N0470_21935) yigL 4515967..4516767 (-) 801 WP_000285362.1 sugar/pyridoxal phosphate phosphatase YigL -
  N0470_RS21945 (N0470_21940) pldB 4516775..4517797 (-) 1023 WP_000487654.1 lysophospholipase L2 -
  N0470_RS21950 (N0470_21945) rhtB 4517908..4518528 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  N0470_RS21955 (N0470_21950) rhtC 4518590..4519210 (-) 621 WP_000928824.1 threonine export protein RhtC -
  N0470_RS21960 (N0470_21955) recQ 4519274..4521103 (-) 1830 WP_000035581.1 ATP-dependent DNA helicase RecQ Machinery gene
  N0470_RS21965 (N0470_21960) pldA 4521236..4522105 (-) 870 WP_001259700.1 phospholipase A -
  N0470_RS21970 (N0470_21965) yigI 4522270..4522737 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  N0470_RS21975 (N0470_21970) rarD 4522789..4523679 (+) 891 WP_000339104.1 EamA family transporter RarD -
  N0470_RS21980 (N0470_21975) yigG 4523774..4524154 (+) 381 WP_000944218.1 protein YigG -
  N0470_RS21985 (N0470_21980) yigF 4524168..4524548 (+) 381 WP_000032581.1 DUF2628 domain-containing protein -
  N0470_RS21990 (N0470_21985) corA 4524591..4525541 (-) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  N0470_RS21995 (N0470_21990) ysgD 4525693..4525749 (-) 57 WP_211180520.1 protein YsgD -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68363.51 Da        Isoelectric Point: 7.2544

>NTDB_id=628246 N0470_RS21960 WP_000035581.1 4519274..4521103(-) (recQ) [Escherichia coli strain THB42-F1]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=628246 N0470_RS21960 WP_000035581.1 4519274..4521103(-) (recQ) [Escherichia coli strain THB42-F1]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCAGGCATT
ATCTACTGCAACAGCCGCGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCCGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391