Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N1707_RS03705 Genome accession   NZ_CP104112
Coordinates   780362..780802 (-) Length   146 a.a.
NCBI ID   WP_000360896.1    Uniprot ID   -
Organism   Escherichia coli strain USDA-ARS-USMARC-51688     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 775362..785802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N1707_RS03680 (N1707_03680) coaE 775852..776472 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  N1707_RS03685 (N1707_03685) - 776497..776541 (+) 45 WP_120795372.1 protein YacM -
  N1707_RS03690 (N1707_03690) guaC 776697..777740 (+) 1044 WP_001217351.1 GMP reductase -
  N1707_RS03695 (N1707_03695) hofC 777775..778977 (-) 1203 WP_050010183.1 protein transport protein HofC -
  N1707_RS03700 (N1707_03700) pilB 778967..780352 (-) 1386 WP_001025156.1 type II secretion system protein GspE Machinery gene
  N1707_RS03705 (N1707_03705) pilA 780362..780802 (-) 441 WP_000360896.1 prepilin peptidase-dependent pilin Machinery gene
  N1707_RS03710 (N1707_03710) nadC 781005..781898 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N1707_RS03715 (N1707_03715) ampD 781986..782537 (+) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N1707_RS03720 (N1707_03720) ampE 782534..783388 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N1707_RS03725 (N1707_03725) aroP 783431..784804 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15647.84 Da        Isoelectric Point: 4.3938

>NTDB_id=627082 N1707_RS03705 WP_000360896.1 780362..780802(-) (pilA) [Escherichia coli strain USDA-ARS-USMARC-51688]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQEILNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=627082 N1707_RS03705 WP_000360896.1 780362..780802(-) (pilA) [Escherichia coli strain USDA-ARS-USMARC-51688]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAATTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363