Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY52_RS02025 Genome accession   NZ_CP087166
Coordinates   459431..460495 (+) Length   354 a.a.
NCBI ID   WP_258677990.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-051     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454431..465495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY52_RS02015 (LOY52_02015) - 455285..456553 (+) 1269 WP_003220889.1 malic enzyme-like NAD(P)-binding protein -
  LOY52_RS02020 (LOY52_02020) - 456767..459211 (-) 2445 WP_408980432.1 penicillin-binding protein 1A -
  LOY52_RS02025 (LOY52_02025) pilM 459431..460495 (+) 1065 WP_258677990.1 pilus assembly protein PilM Machinery gene
  LOY52_RS02030 (LOY52_02030) - 460495..461061 (+) 567 WP_258677991.1 PilN domain-containing protein -
  LOY52_RS02035 (LOY52_02035) pilO 461058..461681 (+) 624 WP_027611313.1 type 4a pilus biogenesis protein PilO -
  LOY52_RS02040 (LOY52_02040) - 461678..462205 (+) 528 WP_027611314.1 pilus assembly protein PilP -
  LOY52_RS02045 (LOY52_02045) pilQ 462219..464297 (+) 2079 WP_258677992.1 type IV pilus secretin PilQ Machinery gene
  LOY52_RS02050 (LOY52_02050) aroK 464302..464820 (+) 519 WP_027611316.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38111.67 Da        Isoelectric Point: 4.8154

>NTDB_id=627082 LOY52_RS02025 WP_258677990.1 459431..460495(+) (pilM) [Pseudomonas sp. B21-051]
MLGLFKKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFSLLATQLAASQERLTVAVVDIGATMTTLSVLNNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627082 LOY52_RS02025 WP_258677990.1 459431..460495(+) (pilM) [Pseudomonas sp. B21-051]
GTGCTGGGACTCTTCAAGAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGTCAGGGCGACCGTTACCGGGTCGAGGCCTACGCGGTAGAGCCGCTGCCGCCAAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAAGGCGTGGGCCATGCCTTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCCGCGGTGATCACCAAGATCATCGAGATGGACGCCGGGCTGACCGATGACGAGCTGGAAAACCAGCT
GAAGATCGAAGCCGACCAATACATTCCCTATCCACTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTGTCGCCGC
GCAACCCGGAGCGGGTCAGCGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTGGCGCTG
GCCGGGCTGACCGCGCGCGTGGTCGATGTCGAGGCCTATGCGCTGGAGCGCTCGTTCAGTCTGCTCGCTACTCAACTGGC
AGCCTCGCAGGAGCGCCTGACCGTGGCGGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGAATAACGGCC
GCATCATCTATACCCGTGAACAGCTGTTCGGTGGCCGGCAACTGACCGAGGAGATCCAGCGCCGCTACGGCCTGACCGTC
GAGCAGGCAGGGCTGGCGAAAAAGCAGGGCGGCCTGCCGGATGACTATGTCAGCGAGGTATTGCAGCCGTTCCGTGATGC
TCTGGTGCAGCAGGTCTCGCGCTCGTTGCAGTTCTTCTTTGCCTCCGGGCAGTACAACGCCGTCGATCACATTCTGCTGG
CCGGCGGCACCGCCTCGGTGCCGGGGCTGGATCGGCTGATCGAACAGCGTCTGGGCACCCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGAGCCCTGGCCAGTGACGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.65

100

0.557
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483