Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LN047_RS00670 Genome accession   NZ_CP087159
Coordinates   142025..143470 (+) Length   481 a.a.
NCBI ID   WP_373980918.1    Uniprot ID   -
Organism   Achromobacter sp. JD417     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137025..148470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LN047_RS00655 (LN047_00655) - 137557..138885 (+) 1329 WP_373980916.1 amidase -
  LN047_RS00660 (LN047_00660) leuA 139121..140830 (-) 1710 WP_062840150.1 2-isopropylmalate synthase -
  LN047_RS00665 (LN047_00665) - 141349..142026 (-) 678 WP_373980917.1 hypothetical protein -
  LN047_RS00670 (LN047_00670) rpoN/rpoN1 142025..143470 (+) 1446 WP_373980918.1 RNA polymerase factor sigma-54 Machinery gene
  LN047_RS00675 (LN047_00675) - 143592..143870 (+) 279 WP_056327752.1 bacterioferritin-associated ferredoxin -
  LN047_RS00680 (LN047_00680) - 143900..144763 (-) 864 WP_373980919.1 LysR substrate-binding domain-containing protein -
  LN047_RS00685 (LN047_00685) - 144896..145807 (+) 912 WP_056327754.1 DMT family transporter -
  LN047_RS00690 (LN047_00690) bfr 146201..146677 (+) 477 WP_056327755.1 bacterioferritin -
  LN047_RS00695 (LN047_00695) - 146771..147445 (-) 675 WP_056327756.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52631.95 Da        Isoelectric Point: 4.7656

>NTDB_id=627009 LN047_RS00670 WP_373980918.1 142025..143470(+) (rpoN/rpoN1) [Achromobacter sp. JD417]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLDREDEPATAEDRAESERESSVQDDEP
AAERETAVDEMPGSGGVYPDDDASLPEAARPDTLREHLLGQLALTRAAPRDAALASLLIDELDENGYLGSPLDEILGWLP
AEMEPDMDELRAALSLLQSFDPVGIGARDMAECLQLQLRNPDLTLLPEAADPVVLACARRICAEHLPLLATGNAARLCAA
VGCDETTFRAAHSLILRLEPRPGRAWTVPAAEYAVPDVIVRKTRRGWQATLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QAQLQQAKWMIRNVEQRFDTILRVSQAIVERQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTEGGDATSATAVQTYIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=627009 LN047_RS00670 WP_373980918.1 142025..143470(+) (rpoN/rpoN1) [Achromobacter sp. JD417]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGGCCTGGCCAGCACCTGACGCTGACCCCACAACTTCA
GCAATCGATCCGATTGTTGCAGTTGTCCACGCTCGACCTTGAAGCGGAAATCTCGCAAGTGCTGGCCGAGAATCCCCTGC
TGGATCGCGAGGACGAGCCGGCGACGGCGGAGGATCGCGCCGAATCCGAGCGCGAATCTTCGGTTCAGGACGACGAACCG
GCGGCCGAGCGCGAGACCGCCGTCGACGAGATGCCTGGATCGGGCGGGGTGTATCCCGACGACGACGCCAGCCTGCCCGA
GGCGGCGCGGCCGGACACGCTGCGCGAACATCTGCTGGGTCAGCTTGCCCTGACGCGCGCCGCGCCGCGCGATGCCGCGC
TGGCGTCCCTGCTGATCGACGAACTGGACGAAAACGGCTACCTGGGTTCGCCGCTGGACGAAATCCTGGGTTGGCTCCCT
GCCGAAATGGAGCCCGACATGGACGAGCTGCGCGCCGCCCTGTCGCTTCTGCAATCGTTCGACCCGGTGGGCATCGGCGC
GCGCGACATGGCTGAATGCCTGCAACTGCAACTGCGCAATCCCGATCTCACCTTGCTGCCGGAGGCGGCTGACCCCGTCG
TGCTTGCCTGTGCGCGCCGGATCTGCGCCGAGCACCTGCCGCTGTTGGCAACCGGCAACGCCGCGCGCCTGTGTGCGGCG
GTCGGCTGCGACGAGACCACGTTCCGCGCGGCGCATTCGCTGATCCTGCGCCTGGAACCCCGGCCGGGCCGGGCCTGGAC
CGTGCCGGCGGCCGAGTACGCAGTGCCGGACGTCATCGTCCGCAAGACGCGGCGCGGCTGGCAGGCCACGCTGAATAGCG
CCGCGGTGCCACGGCTGCAGATCAATGGGCTGTACGCCCAGATGCTGGGCAACCAGAAGGAATCCGCCCACGCCGGCCTG
CAGGCCCAGCTGCAGCAGGCCAAATGGATGATCCGCAACGTGGAGCAGCGCTTTGACACGATCCTGCGCGTGTCGCAGGC
CATCGTCGAGCGTCAGACGGCTTTCTTCAGCCAGGGGCCGGCGGCGATGCGTCCGCTTATCCTGAAGGACATCGCCGGCG
AGCTTGGTTTGCATGAATCGACCATTTCGCGGGCCACAACACAGAAGTACATGCTCACTCCGTTCGGCACGCTGGAGCTC
AAGCGCTTCTTTGGAACGGGCGTGTCGACAGAAGGCGGCGACGCCACGTCGGCCACCGCGGTGCAGACCTACATCCGCCA
GATGGTGGCCGACGAGAACCGCGCCAAGCCGTTGTCGGATAGCCAGATTATGCAAAAACTGGCCGATCAGGGGATAGTGA
TTGCGCGCCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATTGCCCCCGCCGCGCTGCGCAAGGCGCAAGCCTCCGCA
CGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.907

100

0.511