Detailed information NTG0062646

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAPNAU_RS06300
• Genome accession: NC_022530
• Coordinates: 1265290..1265904 (+)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis NAU-B3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1260290..1270904

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAPNAU_RS06265 (BAPNAU_1183)	aroE	1260977..1261819 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
BAPNAU_RS06270 (BAPNAU_1184)	yhbY	1261813..1262103 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAPNAU_RS06275 (BAPNAU_1185)	-	1262114..1262683 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BAPNAU_RS06280 (BAPNAU_1186)	yqeK	1262673..1263233 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAPNAU_RS06285 (BAPNAU_1187)	rsfS	1263251..1263607 (+)	357	WP_007408260.1	ribosome silencing factor	-
BAPNAU_RS06290 (BAPNAU_1188)	-	1263604..1264341 (+)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
BAPNAU_RS06295 (BAPNAU_1189)	comER	1264410..1265231 (-)	822	WP_014418420.1	late competence protein ComER	-
BAPNAU_RS06300 (BAPNAU_1190)	comEA	1265290..1265904 (+)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAPNAU_RS06305 (BAPNAU_1191)	-	1265971..1266540 (+)	570	WP_003152868.1	ComE operon protein 2	-
BAPNAU_RS06310 (BAPNAU_1192)	comEC	1266541..1268892 (+)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAPNAU_RS20655 (BAPNAU_1193)	-	1268911..1269045 (-)	135	WP_003152870.1	YqzM family protein	-
BAPNAU_RS21320	-	1269086..1269237 (+)	152	Protein_1202	hypothetical protein	-
BAPNAU_RS06320 (BAPNAU_1194)	holA	1269277..1270318 (+)	1042	Protein_1203	DNA polymerase III subunit delta	-
BAPNAU_RS06325 (BAPNAU_1195)	rpsT	1270335..1270601 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=62646 BAPNAU_RS06300 WP_014418419.1 1265290..1265904(+) (comEA) [Bacillus velezensis NAU-B3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=62646 BAPNAU_RS06300 WP_014418419.1 1265290..1265904(+) (comEA) [Bacillus velezensis NAU-B3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363